Methods of spraying saccharification enzymes and fermentation organisms onto lignocellulosic biomass for hydrolysis and fermentation processes
First Claim
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1. A method of producing a saccharification product from a pretreated biomass composition comprising a prehydrolysate and pretreated biomass solids, the method comprising:
- a) separating the prehydrolysate from the pretreated biomass solids to form a first prehydrolysate liquid;
b) adding one or more saccharification enzymes to the first prehydrolysate liquid to form a second prehydrolysate liquid, under suitable conditions to promote subsequent enzymatic hydrolysis;
c) spraying the second prehydrolysate liquid onto the pretreated biomass solids to form an impregnated biomass composition; and
d) maintaining the impregnated biomass composition under suitable conditions to promote enzymatic hydrolysis of the impregnated biomass to produce the saccharification product.
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Abstract
The present invention provides spray methods of delivering saccharification enzymes, fermentation organisms, and other hydrolysis or fermentation ingredients onto lignocellulosic biomass. The methods reduce the need for mechanical mixing when the biomass solids are undergoing enzymatic hydrolysis, and reduce dilution to allow higher product titers in the hydrolysis and/or fermentation steps.
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Citations
18 Claims
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1. A method of producing a saccharification product from a pretreated biomass composition comprising a prehydrolysate and pretreated biomass solids, the method comprising:
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a) separating the prehydrolysate from the pretreated biomass solids to form a first prehydrolysate liquid; b) adding one or more saccharification enzymes to the first prehydrolysate liquid to form a second prehydrolysate liquid, under suitable conditions to promote subsequent enzymatic hydrolysis; c) spraying the second prehydrolysate liquid onto the pretreated biomass solids to form an impregnated biomass composition; and d) maintaining the impregnated biomass composition under suitable conditions to promote enzymatic hydrolysis of the impregnated biomass to produce the saccharification product. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A method of producing a fermentation product from a pretreated biomass composition comprising a prehydrolysate and pretreated biomass solids, the method comprising:
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a) separating the prehydrolysate from the pretreated biomass solids to form a first prehydrolysate liquid; b) adding one or more saccharification enzymes and one or more fermentation organisms to the first prehydrolysate liquid to form a second prehydrolysate liquid, under suitable conditions to promote subsequent enzymatic hydrolysis; c) spraying the second prehydrolysate liquid onto the pretreated biomass solids to form an impregnated biomass composition; and d) maintaining the impregnated biomass composition under suitable conditions to promote enzymatic hydrolysis of the impregnated biomass composition to form a saccharification product and fermentation of the saccharification product to form the fermentation product.
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Specification