Polymerase chain reaction method, polymerase chain reaction droplet device, and polymerase chain reaction droplet device array
First Claim
1. A polymerase chain reaction (PCR) droplet device, comprising:
- a cooling plate;
a substrate disposed on the cooling plate;
a first containing area disposed on the substrate to contain a liquid comprising an analyzer;
a second containing area disposed on the substrate, and the second containing area surrounding the first containing area to contain a hydrophobic solution, wherein the hydrophobic solution is located at a surface of the liquid;
a heating coil disposed on a center of the first containing area and the heating coil is located inside the first containing area;
at least one conducting wire disposed on the substrate and connected with the heating coil; and
a plurality of sensors disposed around the heating coil to detect temperature changes of the heating coil;
wherein, the analyzer comprises a template, a primer, a deoxyribonucleotide triphosphate (dNTP) and a polymerase;
the at least one conducting wire is applied with an electric current or a voltage to heat the heating coil such that buoyancy in an inside of the liquid is generated, thereby driving the analyzer to move to a top of the inside of the liquid and subsequently to move to a periphery of the inside of the liquid so as to form a thermal cycle; and
the template is amplified by recycling the thermal cycle;
wherein, a metal electrical conductivity of the heating coil is higher than a metal electrical conductivity of the at least one conducting wire.
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Abstract
The present invention discloses a polymerase chain reaction (PCR) method, a PCR droplet device and a PCR droplet device array. The steps of the method comprise that a liquid comprising an analyzer is dropped on the heating coil disposed on the droplet device to form a droplet, then dropping a hydrophobic solution to prevent the droplet from evaporating. When an electric current or a voltage is supplied through at least one conducting wire to heat the heating coil, the inside of the droplet can generate buoyancy to drive the analyzer to move to the top of the inside of the droplet. Subsequently, the analyzer is moved to a periphery of the inside of the droplet so as to form a thermal cycle. Therefore the template is amplified by recycling the thermal cycle.
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Citations
17 Claims
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1. A polymerase chain reaction (PCR) droplet device, comprising:
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a cooling plate; a substrate disposed on the cooling plate; a first containing area disposed on the substrate to contain a liquid comprising an analyzer; a second containing area disposed on the substrate, and the second containing area surrounding the first containing area to contain a hydrophobic solution, wherein the hydrophobic solution is located at a surface of the liquid; a heating coil disposed on a center of the first containing area and the heating coil is located inside the first containing area; at least one conducting wire disposed on the substrate and connected with the heating coil; and a plurality of sensors disposed around the heating coil to detect temperature changes of the heating coil; wherein, the analyzer comprises a template, a primer, a deoxyribonucleotide triphosphate (dNTP) and a polymerase;
the at least one conducting wire is applied with an electric current or a voltage to heat the heating coil such that buoyancy in an inside of the liquid is generated, thereby driving the analyzer to move to a top of the inside of the liquid and subsequently to move to a periphery of the inside of the liquid so as to form a thermal cycle; and
the template is amplified by recycling the thermal cycle;wherein, a metal electrical conductivity of the heating coil is higher than a metal electrical conductivity of the at least one conducting wire. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification