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Polymerase chain reaction method, polymerase chain reaction droplet device, and polymerase chain reaction droplet device array

  • US 8,389,273 B2
  • Filed: 02/10/2010
  • Issued: 03/05/2013
  • Est. Priority Date: 12/29/2009
  • Status: Active Grant
First Claim
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1. A polymerase chain reaction (PCR) droplet device, comprising:

  • a cooling plate;

    a substrate disposed on the cooling plate;

    a first containing area disposed on the substrate to contain a liquid comprising an analyzer;

    a second containing area disposed on the substrate, and the second containing area surrounding the first containing area to contain a hydrophobic solution, wherein the hydrophobic solution is located at a surface of the liquid;

    a heating coil disposed on a center of the first containing area and the heating coil is located inside the first containing area;

    at least one conducting wire disposed on the substrate and connected with the heating coil; and

    a plurality of sensors disposed around the heating coil to detect temperature changes of the heating coil;

    wherein, the analyzer comprises a template, a primer, a deoxyribonucleotide triphosphate (dNTP) and a polymerase;

    the at least one conducting wire is applied with an electric current or a voltage to heat the heating coil such that buoyancy in an inside of the liquid is generated, thereby driving the analyzer to move to a top of the inside of the liquid and subsequently to move to a periphery of the inside of the liquid so as to form a thermal cycle; and

    the template is amplified by recycling the thermal cycle;

    wherein, a metal electrical conductivity of the heating coil is higher than a metal electrical conductivity of the at least one conducting wire.

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