Multisignaling oligomeric or polymeric compositions comprising labeled moieties and binding partners

• US 8,389,701 B2
• Filed: 06/24/2010
• Issued: 03/05/2013
• Est. Priority Date: 04/03/2003
• Status: Expired due to Term

• Current Assignee: Enzo Life Sciences Incorporated (Enzo Biochem Incorporated)
• Original Assignee: Enzo Life Sciences Incorporated (Enzo Biochem Incorporated)
• Inventors: Rabbani, Elazar, Stavrianopoulos, Jannis G., Donegan, James J.
• Primary Examiner(s): Riley, Jezia
• Application Number:
  US12/803,354
• Publication Number:
  US 20110028692A1
• Time in Patent Office: 985 Days
• Field of Search: 536/23.1, 536/25.3, 536/26.6, 536/29.2, 536/55.1, 536/123.1, 435/6, 435/91.1
• US Class Current: 536/23.1
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical

Abstract

The present invention provides multisignal labeling reagents and these are useful in a number of biochemical applications, including the manufacture of biomolecular probes and their use in detecting or amplifying analyte-specific moieties.

Claims

1. A composition of matter comprising an oligomer or polymer comprising

(a) two or more labeled moieties bound or attached to said oligomer or polymer;

and

(b) one or more members of a binding partner pair bound or attached to said oligomer or polymer,

wherein said binding partner pairs are non-nucleic acid, and

wherein said labeled moieties are different from said members of a binding pair.

2. The composition of claim 1, wherein said oligomer or polymer is homopolymeric, heteropolymeric or chimeric.

3. The composition of claim 1 or 2, wherein said oligomer or polymer comprises one or more monomeric subunits comprising an amino acid, a nucleotide, a carbohydrate, a sugar, an aromatic compound, a derivatized organic compound that forms an oligomeric or polymeric moiety, an analogs of any the foregoing, or any combination thereof.

4. The composition of claim 3, wherein one or more of said monomeric subunits have been modified.

5. The composition of claim 3, wherein said oligomer or polymer comprises amino acids, amino acid analogs, or any combination thereof.

6. The composition of claim 3, wherein said oligomer or polymer comprises nucleotides, nucleotide analogs, or any combination thereof.

7. The composition of claim 1, wherein said oligomer or polymer is branched.

8. The composition of claim 1 or 7, wherein said oligomer or polymer comprises a complex formed from more than one oligomer or polymer.

9. The composition of claim 8, wherein said complex comprises two or more nucleic acid strands and said complex is formed through base-pairing or triple strand interactions.

10. The composition of claim 9, wherein at least one of said nucleic acid strands comprises one or more nucleotide analogs or modified nucleotides.

11. The composition of claim 1, wherein said oligomer or polymer is linear.

12. The composition of claim 6, wherein the oligomer or polymer comprises a nucleic acid, an abasic nucleic acid, a peptide nucleic acid or a combination of any of the foregoing.

13. The composition of claim 1, wherein said one or more members of a pair of binding partners are bound or attached to the oligomer or polymer through a linker.

14. The composition of claim 13, wherein said linker comprises one or more carbon, nitrogen, oxygen, phosphorus or sulfur atoms in any combination.

15. The composition of claim 13, wherein said linker comprises one or more peptide bonds, alkyl chains, alkene groups, alkyl groups, aryl groups, conjugated systems, sugars or derivatives thereof.

16. The composition of claim 1, wherein said labels provide a directly detectable signal.

17. The composition of claim 16, wherein said direct signal providing labels comprise a fluorescent compound, a phosphorescent compound, a chemiluminescent compound, a chromogenic compound, a chelating compound, an electron dense compound, a magnetic compound, an energy transfer compound, an intercalating compound, or a combination of any of the foregoing.

18. The composition of claim 1, wherein said labels provide an indirectly detectable signal.

19. The composition of claim 18, wherein said indirect signal providing labels comprise an antibody, an antigen, a hapten, a receptor, a hormone, a ligand, an enzyme, or a combination of any of the foregoing.

20. The composition of claim 1, wherein said one or more members of a binding partner paircomprise a sugara lectin, an antigen, an antibody, a ligand, a ligand receptor, a hormone, a hormone receptor, an enzyme, an enzyme substrate, biotin, avidin, streptavidin, digoxigenin, anti-digoxygenin, or a combination of any of the foregoing.

21. The composition of claim 1, wherein said oligomer or polymer comprises one or more charged groups.

22. The composition of claim 21, wherein said one or more charged groups comprise phosphate, carboxylic, sulfone, amine, hydroxyl, or a combination of any of the foregoing.

23. The composition of claim 21, wherein said one or more charged groups are an inherent part of said oligomer or polymer.

24. The composition of claim 21, wherein said one or more charged groups are a non-inherent part of said oligomer or polymer.

25. The composition of claim 1, further comprising one or more target molecules bound or attached to said oliqomer or polymer.

26. The composition of claim 25, wherein said one or more target molecules comprise a peptide, a protein, an antibody, an enzyme, an enzyme substrate, a ligand, a hormone, a receptor, an antigen, a hapten, a lectin, avidin, streptavidin, a toxin, a carbohydrate, an oligosaccharide, a polysaccharide, a ribonucleotide, a deoxyribonucleotide, a dideoxyribonucleotides, an analogue of a deoxynucleotides, a ribonucleotide and a dideoxynucleotides, a modified deoxynucleotides, a modified ribonucleotide, a modified dideoxynucleotide an oligonucleotide, a polynucleotide, an analyte specific moiety, or a combination of any of the foregoing.

27. The composition of claim 25, wherein said one or more target molecules molecules comprise a second member of the binding partner pair, wherein said second member of the binding partner pair is bound to said one or more members of a binding partner pair on said oligomer or polymer, wherein said binding partner pair comprises a sugar/lectin, an antigen/antibody, a hapten/antibody, a ligand/receptor, a hormone/receptor, an enzyme/substrate, biotin/avidin, biotin/streptavidin, digoxygenin/anti-digoxygenin, or any combination of any of the foregoing.

28. The composition of claim 25, wherein said one or more target molecules are chemically linked to said composition.

29. The composition of claim 25, wherein said one or more target molecules comprise analytes or analyte-specific moieties.

30. The composition of claim 27, wherein said one or more members of a binding partner pair comprise:

a sugar and said second member of the target binding partner pair comprises a lectin that binds said sugar,

an antigen and said second member of the target binding partner pair comprises an antibody that binds said antigen,

a ligand and said second member of the target binding partner pair comprises a receptor of said ligand,

a hormone and said second member of the target binding partner pair comprises a receptor of said hormone,

an enzyme and said second member of the target binding partner pair comprises a substrate for said enzyme,

biotin and said second member of the target binding partner pair comprises avidin,

biotin said second member of the target binding partner pair comprises streptavidin, or digoxygenin said second member of the target binding partner pair comprises anti-digoxygenin.

31. The composition of claim 27, wherein said one or more members of a binding partner pair comprise:

a lectin and said second member of the target binding partner pair comprises a sugar that binds to said lectin,

a antibody and said second member of the target binding partner pair comprises an antigen that binds to said antibody,

a receptor and said second member of the target binding partner pair comprises a ligand that binds to said receptor,

a receptor and said second member of the target binding partner pair comprises a hormone that binds to said receptor,

an enzyme substrate and said second member of the target binding partner pair comprises the enzyme,

avidin and said second member of the target binding partner pair comprises biotin,

streptavidin and said second member of the target binding partner pair comprises biotin, and

anti-digoxygenin and said second member of the target binding partner pair comprises digoxygenin.

32. The composition of claim 25, wherein the target molecule further comprises a second member of the binding partner pair such that the second member of the binding partner pair is bound to the member of a binding partner pair bound or attached to said oligomer or polymer binding the oligomeric or polymeric target molecule to the oligomer or polymer.

33. The composition of claim 32, wherein the oligomer or polymer comprises nucleotides or nucleotide analogs and the target molecule comprises amino acids.