Multi-oligomeric or multi-polymeric compositions comprising labeled moieties and binding partners

• US 8,389,702 B2
• Filed: 06/24/2010
• Issued: 03/05/2013
• Est. Priority Date: 04/03/2003
• Status: Expired due to Term

• Current Assignee: Enzo Life Sciences Incorporated (Enzo Biochem Incorporated)
• Original Assignee: Enzo Life Sciences Incorporated (Enzo Biochem Incorporated)
• Inventors: Rabbani, Elazar, Stavrianopoulos, Jannis G., Donegan, James J.
• Primary Examiner(s): Riley, Jezia
• Application Number:
  US12/803,355
• Publication Number:
  US 20100324268A1
• Time in Patent Office: 985 Days
• Field of Search: 536/23.1, 536/25.3, 536/26.6, 536/29.2, 536/55.1, 536/123.1, 435/6, 435/91.1
• US Class Current: 536/23.1
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical

Abstract

The present invention provides multisignal labeling reagents and these are useful in a number of biochemical applications, including the manufacture of biomolecular probes and their use in detecting or amplifying analyte-specific moieties.

Claims

1. A composition of matter comprising:

(a) a first oligomer or polymer comprising one or more first members of a binding partner pair bound or attached thereto; and

(b) at least one second oligomer or polymer bound or attached to said first oligomer or polymer, said at least one second oligomer or polymer comprising two or more labeled moieties bound or attached to said at least one second oligomer or polymer,

wherein said one or more members of a binding partner pair comprise a sugar, a lectin, an antigen, an antibody, a ligand, a receptor, a hormone, a hormone receptor, an enzyme, an enzyme substrate, biotin, avidin, streptavidin, digoxigenin, anti-digoxigenin, or a combination of any of the foregoing.

2. The composition of claim 1, wherein said first oligomer or polymer or said at least one second oligomer or polymer, or both, are homopolymeric, heteropolymeric or chimeric.

3. The composition of claim 1 or 2, wherein said first oligomer or polymer or said at least one second oligomer or polymer, or both, comprise one or more monomeric subunits comprising an amino acid, a nucleotide, a carbohydrate, a sugar, an aromatic compound, a derivatized organic compound that forms an oligomeric or polymeric moiety, an analog of any the foregoing, or any combination thereof.

4. The composition of claim 3, wherein one or more of said monomeric subunits have been modified.

5. The composition of claim 3, wherein said first oligomer or polymer or said at least one second oligomer or polymer, or both, comprise amino acids, amino acid analogs, or any combination thereof.

6. The composition of claim 3, wherein said first oligomer or polymer, or said at least one second oligomer or polymer, or both, comprise nucleotides, nucleotide analogs, or any combination thereof.

7. The composition of claim 3, wherein said first oligomer or polymer and said at least one second oligomer or polymer comprise different monomeric subunits.

8. The composition of claim 1, wherein said first oligomer or polymer or said at least one second oligomer or polymer, or both, are branched.

9. The composition of claim 1, wherein said first oligomer or polymer and said at least one second oligomer or polymer comprise nucleic acid strands joined through base-pairing.

10. The composition of claim 1 wherein said first oligomer or polymer or said at least one second oligomer or polymer, or both, are linear.

11. The composition of claim 3, wherein said first oligomer or polymer and said at least one second oligomer or polymer comprise the same monomeric subunits.

12. The composition of claim 6, wherein at least one of said first oligomer or polymer, or said at least one second oligomer or polymer, or both, comprise one or more nucleotide analogs or modified nucleotides.

13. The composition of claim 1, wherein said one or more members of a binding partner pair are bound or attached to the first oligomer or polymer through a linker.

14. The composition of claim 13, wherein said linker comprises one or more carbon, nitrogen, oxygen, phosphorus or sulfur atoms in any combination.

15. The composition of claim 13, wherein said linker comprises one or more peptide bonds, alkyl chains, alkene groups, alkyl groups, aryl groups, conjugated systems, sugars or derivatives thereof.

16. The composition of claim 1, wherein said first oligomer or polymer further comprises two or more labeled moieties bound or attached to said first oligomer or polymer.

17. The composition of claim 1, further comprising two or more second oligomers or polymers independently bound or attached to said first oligomer or polymer, said two or more second oligomers or polymers each comprising two or more labeled moieties wherein the labels are bound or attached to said at least one second oligomer or polymer.

18. The composition of claim 1, wherein said first oligomer or polymer, or said at least one second oligomer or polymer, or both comprise one or more charged groups.

19. The composition of claim 18, wherein said one or more charged groups comprise a phosphate, carboxylic, sulfone, amine or hydroxyl moiety.

20. The composition of claim 18, wherein said one or more charged groups are an inherent part of said oligomer or polymer.

21. The composition of claim 18, wherein said one or more charged groups are a non-inherent part of said oligomer or polymer.

22. The composition of claim 1, further comprising a target molecule bound or attached to said first oligomer or polymer through the first member of the binding partner pair joined to a second member of the binding partner pair.

23. The composition of claim 22, wherein said target molecule comprises a peptide, a protein, an antibody, an enzyme, an enzyme substrate, a ligand, a hormone, a receptor, an antigen, a hapten, a lectin, a carbohydrate, an oligosaccharide, a polysaccharide, a polynucleotide, an analyte specific moiety, or a combination of any of the foregoing.

24. The composition of claim 22, wherein said one or more target molecules comprise amino acids.

25. The composition of claim 22, wherein said one or more first members of a binding partner pair comprise:

a sugar and said second member of the binding partner pair comprises a lectin that binds said sugar,

an antigen and said second member of the binding partner pair comprises an antibody that binds said antigen,

a ligand and said second member of the binding partner pair comprises a receptor of said ligand,

a hormone and said second member of the binding partner pair comprises a receptor of said hormone,

an enzyme and said second member of the binding partner pair comprises a substrate for said enzyme,

biotin and said second member of the binding partner pair comprises avidin,

biotin said second member of the binding partner pair comprises streptavidin, or

digoxigenin said second member of the binding partner pair comprises anti-digoxigenin.

26. The composition of claim 22, wherein said one or more first members of a binding partner pair comprise:

a lectin and said second member of the binding partner pair comprises a sugar that binds to said lectin,

an antibody and said second member of the binding partner pair comprises an antigen that binds to said antibody,

a receptor and said second member of the binding partner pair comprises a ligand that binds to said receptor,

a receptor and said second member of the binding partner pair comprises a hormone that binds to said receptor,

an enzyme substrate and said second member of the binding partner pair comprises the enzyme,

avidin and said second member of the binding partner pair comprises biotin,

streptavidin and said second member of the binding partner pair comprises biotin, and

anti-digoxigenin and said second member of the target binding partner pair comprises digoxigenin.

27. The composition of claim 1, wherein said labels provide a directly detectable signal.

28. The composition of claim 27, wherein said direct signal providing labels comprise a fluorescent compound, a phosphorescent compound, a chemiluminescent compound, a chromogenic compound, a chelating compound, an electron dense compound, a magnetic compound, an energy transfer compound, an intercalating compound, or a combination of any of the foregoing.

29. The composition of claim 1, wherein said labels provide an indirectly detectable signal.

30. The composition of claim 29, wherein said indirect signal providing labels comprise an antibody, an antigen, a hapten, a receptor, a hormone, a ligand, an enzyme, or a combination of any of the foregoing.

31. The composition of claim 22, wherein said first oligomer or polymer and said at least one second oligomer or polymer is a nucleic acid or nucleic acid analog, and said one or more target molecules is a peptide or a protein.