Optical determination of glucose utilizing boronic acid adducts
First Claim
1. A device for optically determining an analyte concentration, which device comprises:
- an analyte permeable component;
a fluorophore confined by, immobilized in, or covalently attached to the analyte permeable component, which fluorophore absorbs light at a first wavelength and emits light at a second wavelength, wherein the fluorophore comprises a substituted pyrene sulfonate;
a quencher confined by, immobilized in, or covalently attached to the analyte permeable component, which quencher modifies the light emitted by the fluorophore by an amount related to the analyte concentration;
a light source; and
a detector.
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Abstract
The present invention concerns an improved optical method and optical sensing device for determining the levels of polyhydroxyl-substituted organic molecules in vitro and/or in vivo in aqueous media. The range of detection is between about 400 and 800 nm. In particular, a sensory devise is implemented in a mammal to determine sugar levels. Specifically, a dye is combined with a conjugated nitrogen-containing heterocyclic aromatic boronic acid-substituted bis-onium compound in the presence of a sugar, such as fructose or glucose. The viologens are preferred as the aromatic conjugated nitrogen-containing boronic acid substituted compounds. The method is useful to determine sugar levels in a human being.
6 Citations
15 Claims
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1. A device for optically determining an analyte concentration, which device comprises:
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an analyte permeable component; a fluorophore confined by, immobilized in, or covalently attached to the analyte permeable component, which fluorophore absorbs light at a first wavelength and emits light at a second wavelength, wherein the fluorophore comprises a substituted pyrene sulfonate; a quencher confined by, immobilized in, or covalently attached to the analyte permeable component, which quencher modifies the light emitted by the fluorophore by an amount related to the analyte concentration; a light source; and a detector. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification