Compositions for use in identification of bacteria

• US 8,394,945 B2
• Filed: 03/07/2007
• Issued: 03/12/2013
• Est. Priority Date: 09/11/2003
• Status: Expired due to Fees

• Current Assignee: Ibis Biosciences Incorporated (Abbott Laboratories)
• Original Assignee: Ibis Biosciences Incorporated (Abbott Laboratories)
• Inventors: Sampath, Rangarajan, Hall, Thomas A., Ecker, David J., Blyn, Lawrence
• Primary Examiner(s): WOOLWINE, SAMUEL C
• Application Number:
  US11/683,286
• Publication Number:
  US 20120122098A1
• Time in Patent Office: 2,197 Days
• Field of Search: None
• US Class Current: 536/24.33
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6858 Allele-specific amplification
• CPC Class Codes: C12Q 1/689 for bacteria
• CPC Class Codes: C12Q 2537/143 Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
• CPC Class Codes: C12Q 2565/627 being a mass spectrometer
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis.

Claims

1. A kit for identifying, determining one or more characteristics of, or detecting a Staphylococcus aureus bioagent comprising a first oligonucleotide primer pair comprising a forward and a reverse primer wherein said forward primer comprises the sequence of SEQ ID NO:

440 and said reverse primer comprises the sequence of SEQ ID NO;

1076 and at least one additional primer pair designed to hybridize to a Staphylococcus aureus gene encoding arcC, aroE, gmk, pta, tpi, yqi, wherein said at least one additional primer pair comprises SEQ ID NOs;

437;

1232, SEQ ID NOs;

590;

891, SEQ ID NOs;

474;

869, SEQ ID NOs;

268;

1284, SEQ ID NOs;

418;

1301, SEQ ID NOs;

318;

1300 or a combination thereof.

2. The kit of claim 1 further comprising at least one other additional primer pair designed to hybridize to a Staphylococcus aureus gene encoding mecA, mecR1, pvluk or a combination thereof.

3. The kit of claim 1, wherein said forward primer of said first oligonucleotide primer pair is SEQ ID NO:

440.

4. The kit of claim 1, wherein said reverse primer of said first oligonucleotide primer pair is SEQ ID NO:

1076.

5. The kit of claim 1, wherein at least one of said forward primer and said reverse primer of said first oligonucleotide primer pair comprises at least one modified nucleobase.

6. The kit of claim 5, wherein said at least one modified nucleobase is a mass modified nucleobase.

7. The kit of claim 6, wherein said mass modified nucleobase is 5-Iodo-C.

8. The kit of claim 6, wherein said mass modified nucleobase comprises a molecular mass modifying tag.

9. The kit of claim 5, wherein said at least one modified nucleobase is a universal nucleobase.

10. The kit of claim 9, wherein said universal nucleobase is inosine.