Method for analysis of pathogenic microorganisms using raman spectroscopic techniques
First Claim
1. A method comprising:
- illuminating a sample using wide-field illumination to thereby produce a first plurality of interacted photons;
assessing the first plurality of interacted photons using a spectroscopic device to thereby generate a Raman data set representative of the sample;
analyzing the Raman data set using a computer to determine at least one of;
the presence of a pathogenic microorganism in the sample and the absence of a pathogenic microorganism in the sample.
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Abstract
A method for assessing the presence of a pathogenic microorganism in a sample. A sample is illuminated to thereby produce a first plurality of interacted photons which may be scattered, emitted, reflected and/or absorbed by the sample. The first plurality of interacted photons are assessed to thereby generate a Raman data set representative of the sample. This Raman data set is analyzed to thereby determine at least one of: the presence of a pathogenic microorganism in said sample and the absence of a pathogenic microorganism in said sample. The Raman data set may comprise at least one of a Raman spectrum and/or a Raman chemical image representative of the sample. The analysis may comprise comparing said Raman data set to at least one reference Raman data set representative of a known sample. This may be achieved using a chemometric technique.
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Citations
23 Claims
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1. A method comprising:
- illuminating a sample using wide-field illumination to thereby produce a first plurality of interacted photons;
assessing the first plurality of interacted photons using a spectroscopic device to thereby generate a Raman data set representative of the sample;
analyzing the Raman data set using a computer to determine at least one of;
the presence of a pathogenic microorganism in the sample and the absence of a pathogenic microorganism in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
- illuminating a sample using wide-field illumination to thereby produce a first plurality of interacted photons;
Specification