Using populations of beads for the fabrication of arrays on surfaces
First Claim
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1. A method of creating an array of features, comprising(a) providing a plurality of beads, wherein each bead in the plurality of beads comprises nucleic acid content;
- (b) contacting the plurality of beads with a surface to produce a layer of beads on the surface;
(c) transferring the nucleic acid content from the beads to the surface to create an array of spatially discrete features on the surface, wherein each spatially discrete feature comprises nucleic acid content from a bead in the plurality of beads; and
(d) performing an amplification reaction using the nucleic acid content at each of the discrete features on the surface, thereby producing a homogenous population of nucleic acids at each feature.
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Abstract
The present invention provides a method of creating an array of features. The method can include steps of (a) providing a plurality of beads, wherein each bead in the plurality of beads includes probe content; (b) contacting the plurality of beads with a surface to produce a layer of beads on the surface; and (c) transferring the probe content from the beads to the surface to create an array of spatially discrete features on the surface, wherein each spatially discrete feature includes probe content from a bead in the plurality of beads.
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27 Claims
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1. A method of creating an array of features, comprising
(a) providing a plurality of beads, wherein each bead in the plurality of beads comprises nucleic acid content; -
(b) contacting the plurality of beads with a surface to produce a layer of beads on the surface; (c) transferring the nucleic acid content from the beads to the surface to create an array of spatially discrete features on the surface, wherein each spatially discrete feature comprises nucleic acid content from a bead in the plurality of beads; and (d) performing an amplification reaction using the nucleic acid content at each of the discrete features on the surface, thereby producing a homogenous population of nucleic acids at each feature. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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Specification