Use of stem-loop oligonucleotides in the preparation of nucleic acid molecules
First Claim
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1. A method of preparing a nucleic acid molecule, comprising:
- providing a double stranded nucleic acid molecule; and
attaching one strand of a stem-loop oligonucleotide comprising an inverted repeat, a non-replicable base or sequence and a loop to the double stranded nucleic acid molecule to produce an oligonucleotide-attached nucleic acid molecule.
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Abstract
The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
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44 Claims
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1. A method of preparing a nucleic acid molecule, comprising:
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providing a double stranded nucleic acid molecule; and attaching one strand of a stem-loop oligonucleotide comprising an inverted repeat, a non-replicable base or sequence and a loop to the double stranded nucleic acid molecule to produce an oligonucleotide-attached nucleic acid molecule. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44)
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Specification