Probe, probe set, probe carrier, and testing method
First Claim
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1. A method of specifically detecting the internal transcribed spacer region (ITS) region of a DNA of Arthroderma vanbreuseghemii which is a pathogenic fungus, comprising:
- (i) reacting a sample with a probe carrier under stringent conditions, wherein the probe carrier has immobilized thereon a probe set comprising two probes arranged at an interval, the two probes consisting of the following base sequences (A) and (B) respectively to specifically detect the DNA of Arthroderma vanbreuseghemii;
(A) tctctttagtggctcaacgctgga (SEQ ID NO.
1) or the fully complementary sequence thereof; and
(B) ggacagacgcaaaaaaattctttcagaag (SEQ ID NO.
2) or the fully complementary sequence thereof; and
(ii) detecting a reaction intensity of each probe on the probe carrier reacted with a nucleic acid contained in the sample to detect the DNA of Arthroderma vanbreuseghemii that reacts with both of the two probes.
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Abstract
A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof.
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4 Claims
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1. A method of specifically detecting the internal transcribed spacer region (ITS) region of a DNA of Arthroderma vanbreuseghemii which is a pathogenic fungus, comprising:
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(i) reacting a sample with a probe carrier under stringent conditions, wherein the probe carrier has immobilized thereon a probe set comprising two probes arranged at an interval, the two probes consisting of the following base sequences (A) and (B) respectively to specifically detect the DNA of Arthroderma vanbreuseghemii;
(A) tctctttagtggctcaacgctgga (SEQ ID NO.
1) or the fully complementary sequence thereof; and(B) ggacagacgcaaaaaaattctttcagaag (SEQ ID NO.
2) or the fully complementary sequence thereof; and(ii) detecting a reaction intensity of each probe on the probe carrier reacted with a nucleic acid contained in the sample to detect the DNA of Arthroderma vanbreuseghemii that reacts with both of the two probes. - View Dependent Claims (2, 3, 4)
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Specification