Crystalline anti-human IL-12 antibodies
First Claim
Patent Images
1. A crystal of an intact IgG anti-human IL-12 antibody comprising a light chain variable region (LCVR) comprising the amino acid sequence of SEQ ID NO:
- 2, and a heavy chain variable region (HCVR) comprising the amino acid sequence of SEQ ID NO;
1, and wherein said crystal has a length of about 2-500 μ
m.
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Abstract
The invention relates to batch crystallization methods for crystallizing an anti-hIL-12 antibody that allows the production of the antibody on an industrial scale, antibody crystals obtained according to the methods, compositions containing the crystals, and methods of using the crystals and the compositions.
101 Citations
49 Claims
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1. A crystal of an intact IgG anti-human IL-12 antibody comprising a light chain variable region (LCVR) comprising the amino acid sequence of SEQ ID NO:
- 2, and a heavy chain variable region (HCVR) comprising the amino acid sequence of SEQ ID NO;
1, and wherein said crystal has a length of about 2-500 μ
m. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
- 2, and a heavy chain variable region (HCVR) comprising the amino acid sequence of SEQ ID NO;
-
34. A batch crystallization method for crystallizing an intact IgG anti-human IL-12 antibody comprising a light chain variable region (LCVR) comprising the amino acid sequence of SEQ ID NO:
- 2, and a heavy chain variable region (HCVR) comprising the amino acid sequence of SEQ ID NO;
1, and wherein said crystal has a length of about 2-500 μ
m, the method comprising the steps of;(a) providing an aqueous crystallization mixture having an aqueous solution of the antibody in admixture with at least one polyalkylene glycol having an average molecular weight in the range of about 400 to about 10,000 as a crystallization agent, wherein the antibody concentration is in the range of about 0.5 to about 280 mg/ml; and (b) incubating the aqueous crystallization mixture at a pH of about pH 4 to about 6.5 and at a temperature of about 4°
C. to about 37°
C. until crystals of the antibody having a length of about 2-500 μ
m are formed. - View Dependent Claims (35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
- 2, and a heavy chain variable region (HCVR) comprising the amino acid sequence of SEQ ID NO;
Specification