Enzymatic signal generation and detection of binding complexes in stationary fluidic chip
First Claim
1. A method of detecting an analyte, comprisingproviding a magnetic affinity complex and a signal affinity complex in a fluidic network comprising a plurality of fluidic zones, wherein the plurality of fluidic zones comprises a sample zone, a cleaning zone, and a detection zone, wherein the fluidic network is functionally coupled to a vibration element, and wherein the signal affinity complex comprises a signal particle comprising a SERS-active nanoparticle,introducing a sample suspected of comprising an analyte into the sample zone, wherein the analyte combines with the magnetic affinity complex and the signal affinity complex to form a sandwich binding complex,activating a microcoil array or a mechanically movable permanent magnet functionally coupled to the fluidic network to thereby move the sandwich binding complex to the detection zone without fluidic movement of a fluid in the plurality of the fluidic zones, wherein the microcoil array comprises a plurality of microcoils arranged in a non-overlapping relationship, anddetecting the presence of the sandwich binding complex within the detection zone using a SERS detection element functionally coupled to the fluidic network.
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Abstract
An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.
21 Citations
32 Claims
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1. A method of detecting an analyte, comprising
providing a magnetic affinity complex and a signal affinity complex in a fluidic network comprising a plurality of fluidic zones, wherein the plurality of fluidic zones comprises a sample zone, a cleaning zone, and a detection zone, wherein the fluidic network is functionally coupled to a vibration element, and wherein the signal affinity complex comprises a signal particle comprising a SERS-active nanoparticle, introducing a sample suspected of comprising an analyte into the sample zone, wherein the analyte combines with the magnetic affinity complex and the signal affinity complex to form a sandwich binding complex, activating a microcoil array or a mechanically movable permanent magnet functionally coupled to the fluidic network to thereby move the sandwich binding complex to the detection zone without fluidic movement of a fluid in the plurality of the fluidic zones, wherein the microcoil array comprises a plurality of microcoils arranged in a non-overlapping relationship, and detecting the presence of the sandwich binding complex within the detection zone using a SERS detection element functionally coupled to the fluidic network.
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13. A method of detecting an analyte, comprising
providing a magnetic affinity complex and a signal analyte complex in a fluidic network comprising a plurality of fluidic zones, wherein the plurality of fluidic zones comprises a sample zone, a cleaning zone, and a detection zone, wherein the fluidic network is functionally coupled to a vibration element, and wherein the signal analyte complex comprises a SERS-active nanoparticle, an optical nanoparticle, a fluorescent nanoparticle, and optionally comprises a catalytic element, introducing a sample suspected of comprising an analyte into the sample zone, wherein the analyte combines with the magnetic affinity complex to form a magnetic binding complex, activating a microcoil array or a mechanically movable permanent magnet functionally coupled to the fluidic network to thereby move the magnetic binding complex within the fluidic zone or to another fluidic zone without fluidic movement of a fluid in the plurality of the fluidic zones, displacing the analyte from the magnetic binding complex with the signal analyte complex to form a competitive binding complex, optionally activating the array of microcoils or mechanically movable permanent magnet to move the competitive binding complex within the fluidic network prior to detecting the competitive binding complex and/or the unbound signal analyte complex, and detecting the presence of the unbound signal analyte complex within the detection zone using a detection element functionally coupled to the fluidic network, wherein the detection element is a optical detection element or an electrical detection element.
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21. A method of detecting an analyte, comprising
providing a coded magnetic affinity complex and a magnetic signal affinity complex in a fluidic network comprising a plurality of fluidic zones, wherein the plurality of fluidic zones comprises a sample zone, a cleaning zone, and a detection zone, wherein the fluidic network is functionally coupled to a vibration element, and wherein the magnetic signal affinity complex comprises a SERS-active nanoparticle, a fluorescent nanoparticle, an optical nanoparticle, a MRI-active nanoparticle, and optionally comprises a catalytic element, introducing a sample suspected of comprising an analyte into the sample zone, wherein the analyte combines with the coded magnetic affinity complex to form a coded magnetic binding complex, activating a microcoil array or a mechanically movable permanent magnet functionally coupled to the fluidic network to thereby move the coded magnetic binding complex to a first affinity surface without fluidic movement of a fluid in the plurality of the fluidic zones, forming a bound coded magnetic binding complex, detaching the code from the bound coded magnetic binding complex, providing a magnetic signal affinity complex, wherein the detached code binds to the magnetic signal particle to form a coded magnetic signal binding complex, activating the microcoil array or mechanically movable permanent magnet to move the coded signal binding complex to the detection zone comprising a second affinity surface, forming a bound coded signal binding complex, and detecting the bound coded signal binding complex within the detection zone using a detection element functionally coupled to the fluidic network.
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31. A method of detecting an analyte, comprising
providing a magnetic signal affinity complex in a fluidic network comprising a plurality of fluidic zones, wherein the plurality of fluidic zones comprises a sample zone, a cleaning zone, and a detection zone, wherein the fluidic network is functionally coupled to a vibration element, and wherein the magnetic signal affinity complex comprises a SERS-active nanoparticle, a fluorescent nanoparticle, and/or comprises a catalytic element, introducing a sample suspected of an analyte into the sample zone, wherein the analyte combines with the magnetic signal affinity complex to form a magnetic signal binding complex, activating a microcoil array or a mechanically movable permanent magnet functionally coupled to the fluidic network to thereby move the magnetic signal binding complex to the detection zone without fluidic movement of a fluid in the plurality of the fluidic zones, detecting the presence of the binding complex within the detection zone using a detection element functionally coupled to the fluidic network, wherein the detection element is a optical detection element or an electrical detection element.
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32. A method of detecting an analyte, comprising
providing a magnetic affinity complex and a signal affinity complex in a fluidic network comprising a plurality of fluidic zones, wherein the plurality of fluidic zones comprises a sample zone, a cleaning zone, and a detection zone, wherein the fluidic network is functionally coupled to a vibration element, and wherein the signal affinity complex comprises a signal particle comprising a SERS-active nanoparticle, introducing a sample suspected of comprising an analyte into the sample zone, wherein the analyte combines with the magnetic affinity complex and the signal affinity complex to form a sandwich binding complex, activating a microcoil array or a mechanically movable permanent magnet functionally coupled to the fluidic network to thereby move the sandwich binding complex to the detection zone without fluidic movement of a fluid in the plurality of the fluidic zones, and detecting the presence of the sandwich binding complex within the detection zone using a SERS detection element functionally coupled to the fluidic network.
Specification