Biosensors and methods of making

• US 8,414,762 B2
• Filed: 06/05/2012
• Issued: 04/09/2013
• Est. Priority Date: 02/28/2006
• Status: Expired due to Fees

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Inventors: Petyt, Adrian, Hector, Simon Andrew
• Primary Examiner(s): Ball, J. Christopher
• Assistant Examiner(s): DIETERLE, JENNIFER M
• Application Number:
  US13/489,160
• Publication Number:
  US 20120318683A1
• Time in Patent Office: 308 Days
• Field of Search: 204/400, 20440301-40315, 204/409, 205/777.5, 205/778, 205/792
• US Class Current: 205/792
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose

Abstract

Devices and methods for determining the concentration of an analyte in a sample of liquid are provided.

Claims

1. A method of providing a sample to a sample chamber of a biosensor, the method comprising:

(a) contacting a side edge of the biosensor with the sample to provide sample through an opening into the sample chamber; and

(b) exposing the sample to sensing chemistry in the sample chamber, wherein the biosensor comprises a first substrate and a second substrate, the sample chamber is positioned between the first substrate and the second substrate, the sample chamber comprising only two openings,

wherein the biosensor comprises an incompressible element positioned in the sample chamber, wherein the incompressible element provides the two openings in the sample chamber, and wherein the incompressible element is a monofilament, a thread, or a yarn, and wherein the first substrate is deformable and deforms due to the incompressible element.

2. The method of claim 1, further comprising:

(a) venting the sample chamber via a second side edge of the biosensor.

3. The method of claim 1, wherein contacting a side edge of the biosensor with the sample to provide sample through an opening into the sample chamber comprises:

(a) filling the sample chamber by capillary forces.

4. The method of claim 1, wherein contacting a side edge of the biosensor with the sample to provide sample through an opening into the sample chamber comprises:

(a) filling the sample chamber by wicking along the incompressible element.

5. The method of claim 1, wherein the incompressible element comprises the sensing chemistry.

6. The method of claim 5, wherein the sensing chemistry is disposed on the incompressible element.

7. The method of claim 5, wherein the sensing chemistry is disposed within the incompressible element.

8. The method of claim 1, wherein the sensing chemistry comprises an enzyme.

9. The method of claim 1, wherein the sensing chemistry comprises a mediator.

10. The method of claim 1, wherein the sample chamber has a volume of no more than about 1 microliter.

11. The method of claim 1, wherein the sample chamber has a volume of no more than about 0.5 microliter.

12. The method of claim 1, wherein the sample chamber has a volume of no more than about 0.1 microliter.

13. The method of claim 1, wherein the sensing chemistry comprises an enzyme and a redox mediator.

14. The method of claim 1, wherein the biosensor comprises a spacer layer.

15. The method of claim 14, wherein the spacer layer comprises an adhesive layer.

16. The method of claim 15, wherein the adhesive layer is a double-sided adhesive tape.

17. The method of claim 15, wherein the adhesive layer is a double-sided adhesive film.

18. The method of claim 1, wherein the sample chamber comprises an electrode arrangement comprising a working electrode and a counter electrode.

19. The method of claim 18, wherein the sample chamber further comprises a reference electrode.

20. The method of claim 18, wherein the sample chamber further comprises an indicator electrode.