Microfluidic reaction apparatus for high throughput screening
First Claim
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1. A mixing arrangement in an elastomeric microfluidic device, the mixing arrangement comprising:
- a) a reaction well having a single inlet from a flow channel;
b) a first valve configured to open and close the flow channel at a position at or near the single inlet into the reaction well;
c) a second valve configured to open and close the flow channel at a position further from the reaction well than the first valve, thereby defining a segment of the flow channel between the first and second valve;
d) a source of a first fluid that is fluidically connected to said segment by way of a first input channel forming a junction with the segment between the first and second valve; and
e) a source of a second fluid that is fluidically connected to said segment by way of a second input channel positioned behind the second valve;
wherein said segment of the flow channel is configured to receive a volume of the first fluid into the segment by blind filling from the first input channel when the first and second valves are closed; and
wherein said reaction well is configured to receive said volume of the first fluid followed by a volume of the second fluid from said segment when the first and second valves are open.
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Abstract
A microfluidic device is described that has a slug mixing arrangement. A reaction well has an input flow channel with a first valve near the reaction well, and a second valve further from the reaction well. A fluid source is connected to the segment in between the two valves. A second fluid source is connected behind the second valve. The channel between the two valves receives the first fluid by blind filling when the two valves are closed. The reaction well receives the first fluid followed by the second fluid when the first and second valves are open.
266 Citations
24 Claims
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1. A mixing arrangement in an elastomeric microfluidic device, the mixing arrangement comprising:
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a) a reaction well having a single inlet from a flow channel; b) a first valve configured to open and close the flow channel at a position at or near the single inlet into the reaction well; c) a second valve configured to open and close the flow channel at a position further from the reaction well than the first valve, thereby defining a segment of the flow channel between the first and second valve; d) a source of a first fluid that is fluidically connected to said segment by way of a first input channel forming a junction with the segment between the first and second valve; and e) a source of a second fluid that is fluidically connected to said segment by way of a second input channel positioned behind the second valve; wherein said segment of the flow channel is configured to receive a volume of the first fluid into the segment by blind filling from the first input channel when the first and second valves are closed; and wherein said reaction well is configured to receive said volume of the first fluid followed by a volume of the second fluid from said segment when the first and second valves are open. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A microfluidic device comprising a matrix of unit cells arranged in columns and rows;
- wherein each unit cell comprises;
a) a reaction well with a flow channel connected thereto; b) a first valve configured to open and close the flow channel at a position at or near its connection to the reaction well; c) a second valve configured to open and close the flow channel at a position further from the reaction well than the first valve, thereby defining a segment of the flow channel between the first and second valve; d) a source of a first fluid that is fluidically connected to said segment by way of a first input channel forming a junction with the segment between the first and second valve, wherein said segment of the flow channel is configured to receive a volume of the first fluid into the segment by blind filling from the first input channel when the first and second valves are closed; and e) a source of a second fluid that is fluidically connected to said segment by way of a second input channel positioned behind the second valve, wherein each unit cell is configured so that the reaction well can receive a volume of the first fluid followed by a volume of the second fluid from said segment by blind filling through a single open channel into the reaction well when the first and second valves are open; wherein each column of unit cells in the device is fluidically connected to a source of a first fluid by way of a first common input channel; and wherein each row of unit cells in the device is fluidically connected to a source of a second fluid by way of a second common input channel. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21)
- wherein each unit cell comprises;
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22. A microfluidic device comprising a matrix of unit cells arranged in columns and rows;
wherein each unit cell comprises; a) a reaction well with a flow channel connected thereto; b) a first valve configured to open and close the flow channel at a position at or near its connection to the reaction well; c) a second valve configured to open and close the flow channel at a position further from the reaction well than the first valve, thereby defining a segment of the flow channel between the first and second valve; d) a source of a first fluid that is fluidically connected to said segment by way of a first input channel forming a junction with the segment between the first and second valve, wherein said segment of the flow channel is configured to receive a volume of the first fluid into the segment by blind filling from the first input channel when the first and second valves are closed; and e) a source of a second fluid that is fluidically connected to said segment by way of a second input channel positioned behind the second valve, wherein said reaction well is configured to receive said volume of the first fluid followed by a volume of the second fluid from said segment when the first and second valves are open; wherein each column of unit cells in the device is fluidically connected to a source of a first fluid by way of a first common input channel; wherein each row of unit cells in the device is fluidically connected to a source of a second fluid by way of a second common input channel; and wherein said segment of the flow channel in each unit forms one or more additional junctions with additional input channels. - View Dependent Claims (23, 24)
Specification