Multiplexed electrochemical detection system and method
First Claim
1. An electrochemical detection system comprising:
- a multiplexer configured to removably couple to a sample array, the multiplexer configured to selectively apply a first electric potential to a working electrode in each of a plurality of sample locations each comprising a plurality of working electrodes, wherein a single electrical connection between the multiplexer and the sample array couples one of the working electrodes in each of the plurality of sample locations to a first electric potential, the multiplexer further configured to selectively apply a second electric potential to a counter electrode; and
an analyzer coupled to the multiplexer and configured to electrochemically detect a probe hybridization based in part on the first electric potential and the second electric potential.
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Accused Products
Abstract
Multiplexed analysis of molecular structures of samples. A plurality of sample wells is arranged on a substrate. A plurality of electrodes is fabricated on a first side of the substrate. The electrodes are disposed on the side of the substrate exposed to the sample wells. The electrodes include working electrodes, counter electrodes, and optionally include reference electrodes. At least two of the sample wells includes a plurality of working electrodes. The plurality of electrodes is configured to allow electrochemical analysis of the associated sample wells in a multiplexed fashion. The plurality of electrodes is electrically coupled to an interface to a sample analysis system. The interface to the sample analysis system can include contacts or connections. The sample analysis system controls a signal to the electrodes in a multiplexed fashion and performs the electrochemical analysis.
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Citations
10 Claims
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1. An electrochemical detection system comprising:
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a multiplexer configured to removably couple to a sample array, the multiplexer configured to selectively apply a first electric potential to a working electrode in each of a plurality of sample locations each comprising a plurality of working electrodes, wherein a single electrical connection between the multiplexer and the sample array couples one of the working electrodes in each of the plurality of sample locations to a first electric potential, the multiplexer further configured to selectively apply a second electric potential to a counter electrode; and an analyzer coupled to the multiplexer and configured to electrochemically detect a probe hybridization based in part on the first electric potential and the second electric potential. - View Dependent Claims (2, 3, 4, 10)
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5. A kit for performing an assay, comprising:
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a top plate comprising a plurality of open-bottomed wells; a substrate having zones adapted to match up to the wells of the top plate such that said zones on the substrate can be matched with bottom openings of wells of the top plate to close said openings; a plurality of working electrodes positioned on said substrate in each of the zones, the working electrodes each being adapted to have at least one polymer probe attached thereto; a first electrical connection between a first of the plurality of working electrodes in each of the zones, the first electrical connection being configured for interfacing with an analyzer; and a second electrical connection between a second of the plurality of working electrodes in each of the zones, the second electrical connection being configured for interfacing with the analyzer. - View Dependent Claims (6, 7, 8, 9)
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Specification