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Compositions and methods for producing isoprene free of C5 hydrocarbons under decoupling conditions and/or safe operating ranges

  • US 8,420,360 B2
  • Filed: 07/01/2009
  • Issued: 04/16/2013
  • Est. Priority Date: 07/02/2008
  • Status: Active Grant
First Claim
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1. A method of producing isoprene, the method comprising(a) culturing recombinant cells comprising a heterologous nucleic acid encoding an isoprene synthase polypeptide under suitable culture conditions for the production of isoprene in a gas phase;

  • and(b) producing isoprene in the gas phase, wherein the gas phase comprises a nonflammable concentration of isoprene and less than or about 9.5% (volume) oxygen, and wherein the cells produce greater than about 400 nmole/gwcm/hr of isoprene.

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