Compositions and methods for producing isoprene free of C5 hydrocarbons under decoupling conditions and/or safe operating ranges
First Claim
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1. A method of producing isoprene, the method comprising(a) culturing recombinant cells comprising a heterologous nucleic acid encoding an isoprene synthase polypeptide under suitable culture conditions for the production of isoprene in a gas phase;
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Abstract
The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a non-flammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.
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Citations
28 Claims
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1. A method of producing isoprene, the method comprising
(a) culturing recombinant cells comprising a heterologous nucleic acid encoding an isoprene synthase polypeptide under suitable culture conditions for the production of isoprene in a gas phase; - and
(b) producing isoprene in the gas phase, wherein the gas phase comprises a nonflammable concentration of isoprene and less than or about 9.5% (volume) oxygen, and wherein the cells produce greater than about 400 nmole/gwcm/hr of isoprene. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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