×

Compositions and methods for producing bioactive fusion proteins

  • US 8,420,779 B2
  • Filed: 05/22/2008
  • Issued: 04/16/2013
  • Est. Priority Date: 05/22/2007
  • Status: Expired due to Fees
First Claim
Patent Images

1. A composition of matter of the formula
(F1)a

  • X2 and multimers thereof, wherein;

    F1 is a half-life extending moiety, and a is 1;

    X2 is D-(L)c-(P5)d

    (X3)e, (X4)f

    (P5)d-(L)c-D, or (X4)f

    (P5)d-(L)c-D-(L)g-(P6)h

    (X3)i, whereinc and g are each independently 0 or 1, d and h are 1, and e, f, and i are each independently 0, 1, 2, 3, or 4;

    X3 is -(L)j-(P7), j is 0 or 1;

    X4 is (P8)-(L)k-, k is 0 or 1;

    D is a pharmacologically inactive protein domain of human origin, wherein said pharmacologically inactive protein domain is a SH3 domain that comprises a native human amino acid sequence that is an intracellular proline motif (PxxP) recognition and binding domain, and(i) has a mass of 3 kDa to 20 kDa, and(ii) characteristically forms protein aggregates of less than 10 percent of total mass of protein when suspended without other proteins in a pharmaceutically acceptable formulation buffer of interest not comprising a detergent or chaotropic agent;

    P5, P6, P7 and P8 are each independently a selected pharmacologically active protein of interest 5 to 80 amino acid residues in length; and

    L is in each instance a peptidyl linker.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×