Systems and methods for transfer of ions for analysis

• US 8,421,005 B2
• Filed: 02/19/2010
• Issued: 04/16/2013
• Est. Priority Date: 05/26/2006
• Status: Active Grant

• Current Assignee: Bruker Scientific LLC (Bruker Corporation)
• Original Assignee: IonSense, Inc. (Bruker Corporation)
• Inventors: Musselman, Brian D.
• Primary Examiner(s): Berman, Jack
• Assistant Examiner(s): SAHU, MEENAKSHI S
• Application Number:
  US12/709,157
• Publication Number:
  US 20100140468A1
• Time in Patent Office: 1,152 Days
• Field of Search: 250286-288, 250/281, 250/282, 250/292, 250/294, 250/295, 250/396.R, 250/423.R, 250/424
• US Class Current: 250/281
• CPC Class Codes: H01J 49/0404 Capillaries used for transferring samples or ions electrospray nozzles H01J49/167
• CPC Class Codes: H01J 49/0409 Sample holders or containers containers for retaining a material to be analyzed, B01L3/50, for DNA, C12Q1/6834, for biological materials, G01N33/543
• CPC Class Codes: H01J 49/0459 for solid samples
• CPC Class Codes: H01J 49/16 using surface ionisation, e.g. field-, thermionic- or photo-emission

Abstract

The present invention is a device to restrict the sampling of analyte ions and neutral molecules from surfaces with mass spectrometry and thereby sample from a defined area or volume. In various embodiments of the present invention, a tube is used to sample ions formed with a defined spatial resolution from desorption ionization at or near atmospheric pressures. In an embodiment of the present invention, electrostatic fields are used to direct ions to either individual tubes or a plurality of tubes positioned in close proximity to the surface of the sample being analyzed. In an embodiment of the present invention, wide diameter sampling tubes can be used in combination with a vacuum inlet to draw ions and neutrals into the spectrometer for analysis. In an embodiment of the present invention, wide diameter sampling tubes in combination with electrostatic fields improve the efficiency of ion collection.

Claims

1. A system for analyzing a sample, the system comprising:

an ionizing source for converting molecules of a sample into gas phase ions in a region at about atmospheric pressure;

an ion analysis device; and

an ion transfer member operably coupled to a gas flow generating device, wherein the gas flow generating device produces a gas flow that transfers the gas phase ions through the ion transfer member to an inlet of the ion analysis device.

2. The system according to claim 1, wherein the ions are transferred over a significant distance.

3. The system according to claim 1, wherein the ions are sampled over a defined area.

4. The system according to claim 1, wherein the gas flow generating device is a pump that produces a high flow rate and a low compression ratio.

5. The system according to claim 1, wherein the gas flow generating device is a gas jet of the ionizing source.

6. The system according to claim 1, further comprising an electric focusing lens device operably coupled to the ion transfer member to facilitate transfer of ions to the inlet of the ion analysis device.

7. The system according to claim 6, wherein the electric focusing element facilitates focusing of the ions at the center of the transfer member during the transfer.

8. The system according to claim 1, wherein a distal end of the ion transfer member comprises a plurality of inlets for transferring ions from multiple locations to the inlet of the ion analysis device.

9. The system according to claim 1, wherein the ion transfer member is a tube.

10. The system according to claim 9, wherein the tube is composed of a rigid material.

11. The system according to claim 10, wherein the rigid material is metal or glass.

12. The system according to claim 9, wherein the tube is composed of a flexible material.

13. The system according claim 12, wherein the flexible material is tygon.

14. The system according to claim 1, wherein the ionizing source operates by a technique selected from the group consisting of:

electrospray ionization, nano-electrospray ionization, atmospheric pressure matrix-assisted laser desorption ionization, atmospheric pressure chemical ionization, desorption electrospray ionization and electrospray-assisted laser desorption ionization.

15. The system according to claim 1, wherein the ion analysis device is selected from the group of a mass spectrometer, a handheld mass spectrometer, and an ion mobility ion analysis device.

16. The system according to claim 1, wherein the sample material is of at least one state selected from the group consisting of:

solid phase, liquid phase, and gas phase.

17. The system according to claim 1, wherein the sample is of biological origin.

18. A system for analyzing a sample, the system comprising:

an ionizing source for converting molecules of a sample into gas phase ions in a region at about atmospheric pressure;

an ion analysis device; and

an ion transfer member operably coupled to a gas flow generating device, wherein the gas flow generating device produces a gas flow that collects ions into the gas transfer member and transfers the gas phase ions through the ion transfer member to an inlet of the ion analysis device.

19. A method of analyzing a sample, the method comprising:

ionizing a sample to convert molecules of the sample into gas phase ions in a region at about atmospheric pressure;

providing an ion transfer member coupled to a gas flow generating device to produce a laminar gas flow that transfers the gas phase ions to an inlet of the ion analysis device; and

analyzing the ions.

20. The method according to claim 19, wherein sample is ionized by a technique selected from the group consisting of:

electrospray ionization, nano-electrospray ionization, atmospheric pressure matrix-assisted laser desorption ionization, atmospheric pressure chemical ionization, desorption electrospray ionization and electrospray-assisted laser desorption ionization.