Gated amperometry
First Claim
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1. An amperometric method for determining the concentration of an analyte in a sample, comprising:
- applying an input signal to a sample, the input signal comprising at least 3 duty cycles within 180 seconds and each duty cycle comprising an amperometric excitation and a relaxation,where the input signal has redox intensity of at least 0.01 over a 10 second duration of the input signal, andwhere the relaxations of the at least 3 duty cycles each provide an independent diffusion and analyte reaction time during which the analyte generates measurable species;
measuring an output signal from one of the amperometric excitations having a duration from 0.01 second to 1.5 seconds, the output signal responsive to the measurable species; and
determining a concentration of an analyte in the sample in response to the measured output signal, where the determined concentration is responsive to a rate at which the measurable species is oxidized or reduced by the input signal.
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Abstract
A sensor system, device, and methods for determining the concentration of an analyte in a sample is described. Gated amperometric pulse sequences including multiple duty cycles of sequential excitations and relaxations may provide a shorter analysis time and/or improve the accuracy and/or precision of the analysis. The disclosed gated amperometric pulse sequences may reduce analysis errors arising from the hematocrit effect, variance in cap-gap volumes, non-steady-state conditions, mediator background, under-fill, temperature changes in the sample, and a single set of calibration constants.
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Citations
24 Claims
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1. An amperometric method for determining the concentration of an analyte in a sample, comprising:
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applying an input signal to a sample, the input signal comprising at least 3 duty cycles within 180 seconds and each duty cycle comprising an amperometric excitation and a relaxation, where the input signal has redox intensity of at least 0.01 over a 10 second duration of the input signal, and where the relaxations of the at least 3 duty cycles each provide an independent diffusion and analyte reaction time during which the analyte generates measurable species; measuring an output signal from one of the amperometric excitations having a duration from 0.01 second to 1.5 seconds, the output signal responsive to the measurable species; and determining a concentration of an analyte in the sample in response to the measured output signal, where the determined concentration is responsive to a rate at which the measurable species is oxidized or reduced by the input signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification