High temperature flow-through device for rapid solubilization and analysis

• US 8,426,135 B1
• Filed: 11/30/2011
• Issued: 04/23/2013
• Est. Priority Date: 09/24/2004
• Status: Active Grant

• Current Assignee: National Technology & Engineering Solutions Of Sandia LLC (Honeywell International Inc.)
• Original Assignee: Sandia National Laboratories (Government of the United States of America)
• Inventors: West, Jason A. A., Hukari, Kyle W., Patel, Kamlesh D., Peterson, Kenneth A., Renzi, Ronald F.
• Primary Examiner(s): Chunduru, Prabha
• Application Number:
  US13/308,228
• Time in Patent Office: 510 Days
• Field of Search: None
• US Class Current: 435/6.12
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 7/00 Heating or cooling apparatus autoclaves B01J3/04; Heat insulating devices
• CPC Class Codes: C12M 47/06 Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003
• CPC Class Codes: C12M 47/20 Heating or cooling heating or cooling apparatus for laboratory uses B01L7/00
• CPC Class Codes: C12N 1/066 by physical methods

Abstract

Devices and methods for thermally lysing of biological material, for example vegetative bacterial cells and bacterial spores, are provided. Hot solution methods for solubilizing bacterial spores are described. Systems for direct analysis are disclosed including thermal lysers coupled to sample preparation stations. Integrated systems capable of performing sample lysis, labeling and protein fingerprint analysis of biological material, for example, vegetative bacterial cells, bacterial spores and viruses are provided.

Claims

1. A system for delivering biological material to an analytical device, comprising:

a biological material source;

a thermal lyser comprising an entrance and an exit, the entrance being in fluid communication with the biological material source;

a dye source in fluid communication with the exit of the thermal lyser;

a buffer source in fluid communication with the exit of the thermal lyser; and

an exit capable of being in fluid communication with the analytical device.

2. The system of claim 1, wherein the thermal lyser comprises:

an inner conduit for transporting the biological material, the inner conduit having an entrance and an exit; and

a heater situated exterior to the inner conduit.

3. The system of claim 2, further comprising an outer conduit surrounding the inner conduit.

4. The system of claim 3, wherein the heater includes a heating coil wrapped around the outer conduit.

5. The system of claim 2, wherein the inner conduit comprises a capillary tube or a microchannel.

6. The system of claim 5, wherein the microchannel resides in a first substrate layer and the heater comprises a metal trace formed on a second substrate layer, both the first and second substrate layers being bonded together.

7. The system of claim 2, wherein the heater is capable of heating the biological material to a temperature of at least about 125°

C.