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Composite membrane containing a cross-linked enzyme matrix for a biosensor

  • US 8,426,192 B2
  • Filed: 12/14/2009
  • Issued: 04/23/2013
  • Est. Priority Date: 05/31/2001
  • Status: Active Grant
First Claim
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1. A composite membrane for an enzyme sensor, comprising:

  • an inner membrane layer comprising a restorable polymerizable membrane, the inner membrane layer adapted to function as an interference rejection membrane;

    an outer membrane layer comprising polyurethane, and adapted to be disposed adjacent a flow channel during use; and

    an enzyme layer disposed between and in contact with the inner and the outer membrane layers, said enzyme layer comprising a matrix comprising;

    at least one enzyme selected from the group consisting of creatinase and sarcosine oxidase;

    a cross-linking agent; and

    an enzyme stabilizer selected from the group consisting of polyethyleneimine, polypropyleneimine, poly(N-vinylimidazole), polyallylamine, polyvinylpyridine, polyvinylpyrrolidone, polylysine, and protamine;

    wherein the outer membrane is adapted to control diffusion of an analyte into the enzyme layer.

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