Multimode cells and methods of using them

• US 8,426,804 B2
• Filed: 10/20/2011
• Issued: 04/23/2013
• Est. Priority Date: 02/26/2010
• Status: Active Grant

• Current Assignee: Perkin Elmer Life Sciences
• Original Assignee: Perkinelmer Health Sciences Incorporated (Perkinelmer Incorporated)
• Inventors: Badiei, Hamid, Kahen, Kaveh
• Primary Examiner(s): BERMAN, JACK I
• Application Number:
  US13/277,594
• Publication Number:
  US 20120091331A1
• Time in Patent Office: 551 Days
• Field of Search: 250281-300
• US Class Current: 250/281
• CPC Class Codes: H01J 49/00 Particle spectrometers or separator tubes
• CPC Class Codes: H01J 49/0031 Step by step routines describing the use of the apparatus H01J49/0081 takes precedence
• CPC Class Codes: H01J 49/0045 characterised by the fragmentation or other specific reaction
• CPC Class Codes: H01J 49/005 by collision with gas, e.g. by introducing gas or by accelerating ions with an electric field
• CPC Class Codes: H01J 49/0072 by ion/ion reaction, e.g. electron transfer dissociation, proton transfer dissociation
• CPC Class Codes: H01J 49/0077 specific reactions other than fragmentation
• CPC Class Codes: H01J 49/061 Ion deflecting means, e.g. ion gates
• CPC Class Codes: H01J 49/10 Ion sources; Ion guns
• CPC Class Codes: H01J 49/105 using high-frequency excitation, e.g. microwave excitation, Inductively Coupled Plasma [ICP]
• CPC Class Codes: H01J 49/24 Vacuum systems, e.g. maintaining desired pressures

Abstract

A mass spectrometer is provided that is configurable for operation in both a Kinetic Energy Discrimination (KED) mode and a dynamic reaction cell (DRC) mode. To operate in the KED mode, a collision cell can be filled with a quantity of the inert gas, and an energy barrier can be formed between the collision cell and a downstream mass analyzer. To operate instead in the DRC mode, the collision cell can be filled with a quantity of gas that is reactive with the interferer ions.

Claims

1. A system configured to permit switching of a cell between at least two modes comprising a collision mode and a reaction mode, the system comprising:

a cell configured to receive a collision gas in a collision mode to pressurize the cell and configured to receive a reaction gas in a reaction mode to pressurize the cell, the cell comprising a quadrupole rod set; and

a controller electrically coupled to the quadrupole rod set of the cell and configured to provide a waveform from a voltage source to the quadrupole set to provide a quadrupolar field within the cell, the controller configured to provide an effective voltage from the voltage source to the cell in the collision mode to select ions comprising an energy greater than a barrier energy and an effective voltage from the voltage source in the reaction mode to select ions using mass filtering.

2. The system of claim 1, in which the effective voltage provided to the cell in the collision mode and the reaction mode is an offset voltage.

3. The system of claim 1, in which the system is further configured to permit switching to a vented mode to permit transmission of ions by the cell.

4. The system of claim 1, further comprising a gas manifold coupled to the cell and configured to provide the collision gas in the collision mode and the reaction gas in the reaction mode.

5. The system of claim 1, in which the cell comprises an exit aperture electrically coupled to the controller.

6. The system of claim 5, in which the cell comprises an entrance aperture electrically coupled to the controller.

7. The system of claim 1, in which the controller is configured to switch the cell between the collision mode and the reaction mode by exhausting the cell prior to introduction of a reaction gas into the cell.

8. The system of claim 1, in which the controller is configured to switch the cell between the reaction mode and the collision mode by exhausting the cell prior to introduction of a collision gas into the cell.

9. The system of claim 1, further comprising a mass analyzer coupled to the cell.

10. The system of claim 9, in which the mass analyzer comprises an offset voltage that is more positive than an offset voltage of the cell when the cell is operated in the collision mode.

11. The system of claim 10, in which the mass analyzer comprises an offset voltage that is more negative than an offset voltage of the cell when the cell is operated in the reaction mode.

12. The system of claim 1, further comprising an ionization source coupled to the pressurized cell.

13. The system of claim 12, in which the ionization source is an inductively coupled plasma.

14. The system of claim 1, further comprising an additional cell coupled to the cell, the additional cell configured to receive a collision gas in a collision mode to pressurize the additional cell and configured to receive a reaction gas in a reaction mode to pressurize the additional cell, the additional cell comprising a quadrupole rod set.

15. The system of claim 14, in which the controller is configured to operate at least one of the cell and the additional cell in the reaction mode and to operate the other cell in a vented mode.

16. The system of claim 14, in which the controller is configured to operate at least one of the cell and the additional cell in the collision mode and to operate the other cell in a vented mode.

17. The system of claim 14, in which the controller is configured to operate at least one of the cell and the additional cell in the collision mode and to operate the other cell in a reaction mode.

18. A method of switching a quadrupolar cell between a collision mode and a reaction mode, the method comprising:

introducing a first ion stream into the quadrupolar cell, the cell configured to receive a collision gas in a collision mode to pressurize the cell and configured to receive a reaction gas in a reaction mode to pressurize the cell, the cell comprising a quadrupole rod set operative to provide a quadrupolar field within the cell;

selecting ions comprising an energy greater than a barrier energy from the introduced first ion stream by introducing a collision gas into the cell in the collision mode, the cell comprising a voltage effective to permit selection of the ions comprising the energy greater than the barrier energy;

exhausting the introduced first ion stream and the introduced collision gas from the cell;

introducing a second ion stream into the cell; and

selecting ions using mass filtering from the introduced second ion stream by introducing a reaction gas in the reaction mode, the cell comprising a voltage effective to permit selection of the ions using the mass filtering.

19. The method of claim 18, further comprising:

exhausting the introduced second ion stream and the introduced reaction gas from the cell;

introducing an additional ion stream into the cell; and

selecting ions comprising an energy greater than a barrier energy from the introduced additional ion stream by introducing a collision gas into the cell in the collision mode, the cell comprising a voltage effective to permit selection of the ions comprising the energy greater than the barrier energy.

20. The method of claim 19, further comprising configuring the voltage effective to permit selection of the ions comprising the energy greater than the barrier energy as an offset voltage.

21. The method of claim 20, further comprising providing ions from the cell comprising the offset voltage to a coupled mass analyzer comprising an offset voltage.

22. The method of claim 21, further comprising configuring the offset voltage of the mass analyzer to be more positive than the cell offset voltage when the cell is in the collision mode.

23. The method of claim 21, further comprising configuring the offset voltage of the mass analyzer to be more negative than the cell offset voltage when the cell is in the reaction mode.

24. A method of switching a quadrupolar cell between a reaction mode and a collision mode, the method comprising:

introducing a first ion stream into the quadrupolar cell, the cell configured to receive a collision gas in a collision mode to pressurize the cell and configured to receive a reaction gas in a reaction mode to pressurize the cell, the cell comprising a quadrupole rod set operative to provide a quadrupolar field within the cell;

selecting ions using mass filtering from the introduced first ion stream by introducing a reaction gas in the reaction mode, the cell comprising a voltage effective to permit selection of the ions using the mass filtering;

exhausting the introduced first ion stream and the introduced reaction gas from the cell;

introducing a second ion stream into the cell; and

selecting ions comprising an energy greater than a barrier energy from the introduced second ion stream by introducing a collision gas into the cell in the collision mode, the cell comprising a voltage effective to permit selection of the ions comprising the energy greater than the barrier energy.

25. The method of claim 24, further comprising:

exhausting the introduced second ion stream and the introduced collision gas from the cell;

introducing an additional ion stream into the cell; and

selecting ions using mass filtering from the introduced additional ion stream by introducing a reaction gas in the reaction mode, the cell comprising a voltage effective to permit selection of the ions using the mass filtering.

26. The method of claim 24, further comprising providing ions from the cell to a coupled mass analyzer comprising an offset voltage.

27. The method of claim 26, further comprising configuring the offset voltage of the mass analyzer to be more positive than an offset voltage of the cell when the cell is in the collision mode.

28. The method of claim 26, further comprising configuring the offset voltage of the mass analyzer to be more negative than an offset voltage of the cell when the cell is in the reaction mode.