Method for removing antiplatelet agent and anticoagulant from a platelet composition by diafiltration

• US 8,431,334 B2
• Filed: 11/29/2010
• Issued: 04/30/2013
• Est. Priority Date: 01/12/2006
• Status: Expired due to Fees

• Current Assignee: Biovec Transfusion LLC
• Original Assignee: Biovec Transfusion LLC
• Inventors: Sehgal, Lakshman R.
• Primary Examiner(s): SAUCIER, SANDRA E
• Application Number:
  US12/955,200
• Publication Number:
  US 20110076669A1
• Time in Patent Office: 883 Days
• Field of Search: None
• US Class Current: 435/2
• CPC Class Codes: A01N 1/0226 Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
• CPC Class Codes: A61M 1/0272 Apparatus for treatment of blood or blood constituents prior to or for conservation, e.g. freezing, drying or centrifuging
• CPC Class Codes: A61M 1/0281 Apparatus for treatment of blood or blood constituents prior to transfusion, e.g. washing, filtering or thawing
• CPC Class Codes: A61M 2202/0427 Platelets; Thrombocytes

Abstract

The present invention relates to methods for preserving platelet. In one embodiment, the method includes the steps of mixing platelets with a platelet preservation composition to form a platelet preparation, storing the platelet preparation for a desired period of time, and removing the antiplatelet agent and the anticoagulant from the platelet preparation by diafiltration prior to transfusion of the platelets. The platelet preservation composition comprises an effective amount of an antiplatelet agent and an effective amount of an anticoagulant.

Claims

1. A method for preserving platelets, comprising:

mixing platelets with a platelet preservation composition to form a platelet preparation, said platelet preservation composition comprises an effective amount of a short-to-ultra short acting antiplatelet agent comprising a short-to-ultrashort GPIIb/IIIa inhibitor, and an effective amount of a short-to-ultra short acting anticoagulant comprising a short-to-ultrashort acting factor Xa inhibitor;

storing the platelet preparation for a desired period of time; and

removing the antiplatelet agent and the anticoagulant from the platelet preparation by diafiltration prior to transfusion of the platelets.

2. The method of claim 1, wherein said removing step comprises:

flowing the platelet preparation through a filtering tube comprising a filtering membrane and separating the antiplatelet agent and anticoagulant from the platelet preparation by tangential flow filtration.

3. The method of claim 2, wherein the filter membrane comprises a material selected from the group consisting of regenerated cellulose, cellulose acetate, polyamide, polysulfone, polyethersulfone and combinations thereof.

4. The method of claim 3, wherein the filter membrane comprises polysulfone or polyethersulfone.

5. The method of claim 4, wherein the filter membrane has a pore size ranging from molecular weight cut off of 3000 daltons to 0.5 micron.

6. The method of claim 2, wherein the filtering tube has an inner diameter of at least 0.5 mm.

7. The method of claim 2, wherein the platelet preparation flows through the filtering tube at a flow rate of 150 to 3500 ml/min.

8. The method of claim 2, wherein an extraction liquid is circulated outside the filtering tube in a counter current manner.

9. The method of claim 8, wherein the extraction fluid comprises 0.9% w/v sodium chloride.

10. The method of claim 1, wherein the antiplatelet agent is eptifibatide and wherein the anticoagulant is argatroban.

11. The method of claim 10, wherein the antiplatelet agent is eptifibatide at a final concentration of 50 μ

g/unit of platelet, wherein the anticoagulant is argatroban at a final concentration of 2.5 mg/unit of platelet and wherein said unit comprises between 5×

10⁹ to 3×

10¹¹ platelets.