Method and a system for determination of particles in a liquid sample
First Claim
1. A system for the assessment of at least one quantity parameter and/or at least one quality parameter of biological particles in a liquid analyte material, comprisinga sample compartment having a wall part defining an exposing area, the wall part allowing electromagnetic radiation from a liquid sample in the sample compartment to pass through the wall and to be exposed to the exterior, the volume of the liquid sample from which electromagnetic radiation is exposed onto an array of active detection elements being in the range of 0.01 μ
- l to 20 μ
l,one or more LEDs arranged to transmit light onto the sample,an array of active detection elements, onto which an at least one-dimensional spatial image of said volume can be exposed, the image being one which is detectable as an intensity by individual active detection elements, under conditions which will permit processing of the intensities detected by the array of detection elements during the exposure in such a manner that images of electromagnetic signals from the biological particles are identified as distinct from images of electromagnetic signals from background signals,a lens consisting of one or several elements for focusing an image of electromagnetic signals from at least part of the exposing domain onto the array of detection elements, the spatial representation exposed onto the array of detection elements being subject to such a linear enlargement that the ratio of a linear dimension of the image on the array of detection elements to the original linear dimension in the exposing domain is smaller than 10;
1,at least one monochromatic device for separating electronic radiation into substantially one or more wavelength components or wavebands allowing the system to receive fluorescence, anda computer unit for processing the intensities detected by the detection elements in such a manner that signals from the biological particles are identified as distinct from background signals, and for correlating the results of the processing to the at least one quantity parameter and/or the at least one quality parameter of the liquid analyte material.
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Accused Products
Abstract
A method for the assessment of quantity and quality parameters of biological particles in a liquid analyte material. The method comprises applying a volume of a liquid sample to an exposing domain from which exposing domain electromagnetic signals from the sample in the domain can pass to the exterior, and exposing, onto an array of active detection elements such as CCD-elements, a spatial representation of electromagnetic signals having passed from the domain, the representation being detectable as an intensity by individual active detection elements, under conditions permitting processing of the intensities detected by the array of detection elements during the exposure in such a manner that representations of electromagnetic signals from the biological particles are identified as distinct from representations of electromagnetic signals from background signals.
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Citations
30 Claims
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1. A system for the assessment of at least one quantity parameter and/or at least one quality parameter of biological particles in a liquid analyte material, comprising
a sample compartment having a wall part defining an exposing area, the wall part allowing electromagnetic radiation from a liquid sample in the sample compartment to pass through the wall and to be exposed to the exterior, the volume of the liquid sample from which electromagnetic radiation is exposed onto an array of active detection elements being in the range of 0.01 μ - l to 20 μ
l,one or more LEDs arranged to transmit light onto the sample, an array of active detection elements, onto which an at least one-dimensional spatial image of said volume can be exposed, the image being one which is detectable as an intensity by individual active detection elements, under conditions which will permit processing of the intensities detected by the array of detection elements during the exposure in such a manner that images of electromagnetic signals from the biological particles are identified as distinct from images of electromagnetic signals from background signals, a lens consisting of one or several elements for focusing an image of electromagnetic signals from at least part of the exposing domain onto the array of detection elements, the spatial representation exposed onto the array of detection elements being subject to such a linear enlargement that the ratio of a linear dimension of the image on the array of detection elements to the original linear dimension in the exposing domain is smaller than 10;
1,at least one monochromatic device for separating electronic radiation into substantially one or more wavelength components or wavebands allowing the system to receive fluorescence, and a computer unit for processing the intensities detected by the detection elements in such a manner that signals from the biological particles are identified as distinct from background signals, and for correlating the results of the processing to the at least one quantity parameter and/or the at least one quality parameter of the liquid analyte material. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
- l to 20 μ
Specification