Information acquisition method, information acquisition apparatus and disease diagnosis method

• US 8,436,535 B2
• Filed: 02/24/2010
• Issued: 05/07/2013
• Est. Priority Date: 07/02/2003
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Hashimoto, Hiroyuki, Kuge, Katsuaki, Komatsu, Manabu, Nakamura, Kumi, Ban, Kazuhiro, Imamura, Takeshi, Kobayashi, Shin, Okamoto, Tadashi
• Primary Examiner(s): Shen, Bin
• Application Number:
  US12/712,131
• Publication Number:
  US 20100227308A1
• Time in Patent Office: 1,168 Days
• Field of Search: 250/288, 313/564, 435/23
• US Class Current: 313/564
• CPC Class Codes: G01N 33/4833 of solid biological material, e.g. tissue samples, cell cultures tissue in vivo A61B5/00; cell suspensions G01N33/48735
• CPC Class Codes: G01N 33/6848 Methods of protein analysis involving mass spectrometry

Abstract

An information acquisition method for acquiring information on a target object, that includes a step of promoting ionization of the target object using a substance for promoting ionization of the target object to cause the target object to emit, and a step of acquiring information on the mass of the flew target object using time-of-flight secondary ion mass spectrometry.

Claims

1. A method for acquiring information on an object in a tissue of a living organism, comprising the steps of:

applying a solution containing a digestive enzyme to decompose the object in the tissue specifically to a surface of the tissue so as to obtain the decomposed object;

irradiating the surface of the tissue with an ion beam to ionize the decomposed object after applying the solution containing the digestive enzyme to decompose the object in the tissue specifically; and

acquiring information on the mass of the decomposed object that ionizes using time-of-flight secondary ion mass spectrometry after irradiating the surface of the tissue with an ion beam.

2. The method according to claim 1, wherein the object is selected from the group consisting of a protein, a peptide and a metabolite.

3. The method according to claim 1, wherein the tissue is a section of tissue from a living organism.

4. The method according to claim 1, wherein the target object is a protein molecule within the tissue, and the method further comprising conducting two-dimensional imaging with respect to a distribution state of an abundance of the protein molecule.