Methods for the detection and/or quantification of gram positive bacterial contaminants
First Claim
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1. A method of detecting the presence of Gram-positive bacteria in a sample, the method comprising the steps of:
- contacting the sample with a crustacean hemocyte preparation comprising a lipoteichoic acid reactive material, wherein the lipoteichoic acid reactive material is activatable by lipoteichoic acid such that prophenol oxidase is converted into phenol oxidase when the lipoteichoic acid reactive material is contacted with lipoteichoic acid; and
detecting the presence or absence of phenol oxidase in the sample, thereby to detect the presence or absence of Gram-positive bacteria in the sample.
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Abstract
The invention provides methods and compositions for the detection and/or quantification of a Gram positive bacterial contaminant in a sample. In particular, the invention provides hemocyte-based preparations, methods of making such hemocyte-based preparations, and methods of using such hemocyte-based preparations for the detection and/or quantification of the Gram positive bacterial contaminant.
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Citations
15 Claims
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1. A method of detecting the presence of Gram-positive bacteria in a sample, the method comprising the steps of:
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contacting the sample with a crustacean hemocyte preparation comprising a lipoteichoic acid reactive material, wherein the lipoteichoic acid reactive material is activatable by lipoteichoic acid such that prophenol oxidase is converted into phenol oxidase when the lipoteichoic acid reactive material is contacted with lipoteichoic acid; and detecting the presence or absence of phenol oxidase in the sample, thereby to detect the presence or absence of Gram-positive bacteria in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification