Pasting edge heater

• US 8,440,454 B2
• Filed: 04/27/2011
• Issued: 05/14/2013
• Est. Priority Date: 05/17/2004
• Status: Active Grant

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Shin, Hon Siu, Khoo, Hock Lai
• Primary Examiner(s): Bowers, Nathan
• Assistant Examiner(s): EDWARDS, LYDIA E
• Application Number:
  US13/095,274
• Publication Number:
  US 20110200501A1
• Time in Patent Office: 748 Days
• Field of Search: 4352831-3094
• US Class Current: 435/288.4
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046

Abstract

An apparatus and method for thermal cycling including a pasting edge heater. The pasting edge heater can provide substantial temperature uniformity throughout the retaining elements during thermal cycling by a thermoelectric module.

Claims

1. An apparatus for thermal cycling samples, the apparatus comprising:

a sample retaining element comprising a first surface for receiving a sample containment structure, a second surface opposing the first surface, and a substantially flat edge surface,

wherein the first surface provides a plurality of sample wells, and

wherein the substantially flat edge surface is a third surface located between the first surface and the second surface, and

wherein the sample containment structure provides containment for a plurality of samples; and

an edge heater coupled to the substantially flat edge surface of the sample retaining element,

said edge heater adapted to reduce the temperature non-uniformity (TNU) of the sample retaining element;

wherein the TNU of the sample retaining element is between about 0.25°

C. to about 0.50°

C. within between about 5 seconds to about 10 seconds of achieving a sample retaining element temperature of about 95°

C.

2. The apparatus of claim 1, wherein at least one thermal electric module is in contact with the second surface of the sample retaining element.

3. The apparatus of claim 2, further comprising an excitation light source, an excitation light source adapted to induce light to be emitted by the plurality of samples during thermal cycling and a detector.

4. The apparatus of claim 3, further comprising an excitation light source a detector adapted to collecting the fluorescent light emitted by the plurality of samples.

5. The apparatus of claim 2, wherein the edge heater and the at least one thermoelectric module are separately controlled.

6. The apparatus of claim 1, wherein the sample retaining element provides a thermal mass for heating and cooling the sample containment structure during thermal cycling.

7. The apparatus of claim 1, wherein the edge heater is printed on the substantially flat edge surface of the sample retaining element.

8. The apparatus of claim 1, wherein the edge heater is a resistive heater.

9. The apparatus of claim 1, wherein the coupling comprises adhesive coupling.

10. The apparatus of claim 1, wherein the coupling comprises mechanical coupling.

11. An apparatus for thermal cycling samples, the apparatus comprising:

a sample retaining element comprising a first surface for receiving a sample containment structure, a second surface opposing the first surface, and a substantially flat edge surface,

wherein the first surface provides a plurality of sample wells, and

wherein the substantially flat edge surface is a third surface located between the first surface and the second surface, and

wherein the sample retaining element provides a thermal mass for heating and cooling during thermal cycling; and

an edge heater coupled to the substantially flat edge surface of the sample retaining element,

said edge heater adapted to reduce the temperature nonuniformity (TNU) of the sample retaining element;

wherein the TNU of the sample retaining element is about 0.25°

C. within between about 10 seconds to about 20 seconds of achieving a sample retaining element temperature of about 95°

C.

12. The apparatus of claim 11, wherein at least one thermal electric module is in contact with the second surface of the sample retaining element.

13. The apparatus of claim 12, further comprising an excitation light source, an excitation light source adapted to induce light to be emitted by the plurality of samples during thermal cycling and a detector.

14. The apparatus of claim 13, further comprising an excitation light source a detector adapted to collecting the fluorescent light emitted by the plurality of samples.

15. The apparatus of claim 12, wherein the edge heater and the at least one thermoelectric module are separately controlled.

16. The apparatus of claim 11, wherein the edge heater is printed on the substantially flat edge surface of the sample retaining element.

17. The apparatus of claim 11, wherein the edge heater is a resistive heater.

18. The apparatus of claim 11, wherein the coupling comprises adhesive coupling.

19. The apparatus of claim 11, wherein the coupling comprises mechanical coupling.

20. An apparatus for thermal cycling samples, the apparatus comprising:

a sample retaining element comprising a first surface for receiving a sample containment structure, a second surface opposing the first surface, and a substantially flat surface,

wherein the first surface provides a plurality of sample wells, and

wherein the second surface is in thermal contact with at least one thermal electric module, and

wherein the substantially flat surface is a third surface located between the first surface and the second surface, and

wherein the sample retaining element provides a thermal mass for heating and cooling during thermal cycling; and

a heater coupled to the substantially flat surface of the sample retaining element, and

said heater adapted to reduce the temperature nonuniformity (TNU) of the sample retaining element.