Methods of purifying anti A β antibodies
First Claim
Patent Images
1. A method for purifying an anti-Aβ
- antibody having an Fc region from a source liquid comprising the antibody and one or more impurities, comprising the steps of;
adsorbing the anti-Aβ
antibody to an Fc binding agent comprising Protein A;
washing the Fc binding agent to which the anti-Aβ
antibody is adsorbed with a buffer solution containing CaCl2 at a concentration of from 0.5 M to 3 M to remove one or more impurities;
recovering the anti-Aβ
antibody from the Fc binding agent by eluting the antibody using an elution buffer having a pH in a range from 2 to 4; and
subjecting the anti-Aβ
antibody recovered from the Fc binding agent to anion exchange chromatography.
0 Assignments
0 Petitions
Accused Products
Abstract
The present application provides methods of purifying Aβ binding proteins having a Fc region, for example, anti-Aβ antibodies or antibody fusions, by adsorbing the Aβ binding protein to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed Aβ binding protein. The present application also features methods of eluting the purified Aβ binding protein as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.
95 Citations
23 Claims
-
1. A method for purifying an anti-Aβ
- antibody having an Fc region from a source liquid comprising the antibody and one or more impurities, comprising the steps of;
adsorbing the anti-Aβ
antibody to an Fc binding agent comprising Protein A;washing the Fc binding agent to which the anti-Aβ
antibody is adsorbed with a buffer solution containing CaCl2 at a concentration of from 0.5 M to 3 M to remove one or more impurities;recovering the anti-Aβ
antibody from the Fc binding agent by eluting the antibody using an elution buffer having a pH in a range from 2 to 4; andsubjecting the anti-Aβ
antibody recovered from the Fc binding agent to anion exchange chromatography. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
- antibody having an Fc region from a source liquid comprising the antibody and one or more impurities, comprising the steps of;
Specification