Algal desaturases

• US 8,440,805 B2
• Filed: 04/27/2012
• Issued: 05/14/2013
• Est. Priority Date: 04/28/2011
• Status: Expired due to Fees

• Current Assignee: Aurora Algae, Incorporated (Reliance Industries Limited)
• Original Assignee: Aurora Algae, Incorporated (Reliance Industries Limited)
• Inventors: Kilian, Oliver, Vick, Bertrand
• Primary Examiner(s): Monshipouri, Maryam
• Application Number:
  US13/458,914
• Publication Number:
  US 20120277417A1
• Time in Patent Office: 382 Days
• Field of Search: 536/23.2, 435/183
• US Class Current: 536/23.2
• CPC Class Codes: C12N 9/0071 acting on paired donors with incorporation of molecular oxygen (1.14)
• CPC Class Codes: C12Y 114/19 with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)

Abstract

Provided herein are exemplary isolated nucleotide sequences encoding polypeptides having desaturase activity, which utilize fatty acids as substrates.

Claims

1. An isolated nucleotide sequence encoding a polypeptide having desaturase activity, the nucleotide sequence having at least 95% sequence identity to SEQ ID NO:

3.

2. The isolated nucleotide sequence of claim 1 wherein the sequence encodes a functionally active desaturase which utilizes a fatty acid as a substrate.

3. The isolated nucleotide sequence of claim 2, wherein the functionally active desaturase comprises amino acids having the sequence set forth as SEQ ID NO:

8 or a sequence at least 95% identical thereto.

10. The isolated nucleotide sequence of claim 1 wherein the sequence is derived from algae.

11. The isolated nucleotide sequence of claim 10 wherein the algae is of genus Nannochloropsis.

4. An isolated nucleotide sequence encoding a polypeptide having desaturase activity, the nucleotide sequence having at least 95% sequence identity to SEQ ID NO:

4.

5. The isolated nucleotide sequence of claim 4 wherein the sequence encodes a functionally active desaturase which utilizes a fatty acid as a substrate.

6. The isolated nucleotide sequence of claim 5, wherein the functionally active desaturase comprises amino acids having the sequence set forth as SEQ ID NO:

9 or a sequence at least 95% identical thereto.

7. An isolated nucleotide sequence encoding a polypeptide having desaturase activity, the nucleotide sequence having at least 95% sequence identity to SEQ ID NO:

5.

8. The isolated nucleotide sequence of claim 7 wherein the sequence encodes a functionally active desaturase which utilizes a fatty acid as a substrate.

9. The isolated nucleotide sequence of claim 8, wherein the functionally active desaturase comprises amino acids having the sequence set forth as SEQ ID NO:

10 or a sequence at least 95% identical thereto.