Optical detection system for monitoring rtPCR reaction
First Claim
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1. An optical multiplexing system for detecting sample components in at least two different sample chambers, the system comprising:
- a first optical unit for detecting a product of a first polymerase chain reaction (PCR), wherein the first optical unit comprises a first light source and a first detector;
a second optical unit for detecting a product of a second polymerase chain reaction (PCR), wherein the second optical unit comprises a second light source and a second detector, wherein the first optical unit and the second optical unit are spatially separated from each other,wherein the system receives at least two sample chambers to be received at positions corresponding to the first and second optical units so that (a) the first and second light sources, respectively, simultaneously illuminate the at least two sample chambers and (b) the first and second detectors, respectively, simultaneously receive light from the at least two sample chambers, said first and second optical units simultaneously performing first and second optical measurement using two different optical wavelengths at said two spatially separated sample chambers, andwherein the system is adapted for a relative movement of the first optical unit and the second optical unit relative to the at least two sample chambers;
a first heater associated with a first sample chamber of the at least two sample chambers, said first heater creating a first temperature progression at the first sample chamber;
a second heater associated with a second sample chamber of the at least two sample chambers, said second heater creating a second temperature progression at the second sample chamber; and
a control unit for receiving a PCR protocol, wherein said control unit controls the first and second heaters according to the PCR protocol to cause real time PCR reactions in the first and second sample chambers, said control unit simultaneously quantifying a targeted and amplified DNA molecule by relative movement of the first optical unit and second optical unit relative to the at least two sample chambers.
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Abstract
An optical detection system for monitoring real-time PCR reactions in a plurality of sample chambers with a plurality of optical units is provided. Due to a relative movement, the optical units are relative to the sample chambers, color multiplexing and space multiplexing are combined for optically detecting pathogens in a sample during the process of the PCR and delivering a quantitative result.
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Citations
19 Claims
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1. An optical multiplexing system for detecting sample components in at least two different sample chambers, the system comprising:
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a first optical unit for detecting a product of a first polymerase chain reaction (PCR), wherein the first optical unit comprises a first light source and a first detector; a second optical unit for detecting a product of a second polymerase chain reaction (PCR), wherein the second optical unit comprises a second light source and a second detector, wherein the first optical unit and the second optical unit are spatially separated from each other, wherein the system receives at least two sample chambers to be received at positions corresponding to the first and second optical units so that (a) the first and second light sources, respectively, simultaneously illuminate the at least two sample chambers and (b) the first and second detectors, respectively, simultaneously receive light from the at least two sample chambers, said first and second optical units simultaneously performing first and second optical measurement using two different optical wavelengths at said two spatially separated sample chambers, and wherein the system is adapted for a relative movement of the first optical unit and the second optical unit relative to the at least two sample chambers; a first heater associated with a first sample chamber of the at least two sample chambers, said first heater creating a first temperature progression at the first sample chamber; a second heater associated with a second sample chamber of the at least two sample chambers, said second heater creating a second temperature progression at the second sample chamber; and a control unit for receiving a PCR protocol, wherein said control unit controls the first and second heaters according to the PCR protocol to cause real time PCR reactions in the first and second sample chambers, said control unit simultaneously quantifying a targeted and amplified DNA molecule by relative movement of the first optical unit and second optical unit relative to the at least two sample chambers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for detecting sample components in at least two different sample chambers, the method comprising the steps of:
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receiving at least two sample chambers, each sample chamber having a sample; aligning a first sample chamber of the at least two sample chambers with a first optical unit comprised of a first light source and a first detector; aligning a second sample chamber of the at least two sample chambers with a second optical unit comprised of a second light source and a second detector, wherein the first optical unit and the second optical unit are spatially separated from each other; simultaneously performing (i) a first optical measurement of a first sample chamber of the at least two sample chambers using a first optical wavelength and (ii) a second optical measurement of a second sample chamber of the at least two sample chambers using a second optical wavelength, wherein simultaneously performing the first and second optical measurements includes; (a) simultaneously illuminating the at least two sample chambers using the first and second light sources, respectively, and (b) simultaneously receiving light from the at least two sample chambers using the first and second detectors, respectively; moving the first optical unit and the second optical unit relative to the at least two sample chambers so as to cause an alignment of the first sample chamber with the second optical unit and an alignment of the second sample chamber with the first optical unit; heating the first sample chamber of the at least two sample chambers with a first heater to create a first temperature progression at the first sample chamber; heating the second sample chamber of the at least two sample chambers with a second heater to create a second temperature progression at the second sample chamber; and controlling the first and second heaters according to a PCR protocol to cause real time PCR reactions in the first and second sample chambers, and simultaneously quantifying a targeted and amplified DNA molecule by relative movement of the first optical unit and the second optical unit relative to the at least two sample chambers. - View Dependent Claims (11, 12)
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13. A computer program element characterized by being adapted, when in use on a general purpose computer, to cause the computer to perform the steps of:
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aligning a first sample chamber with a first optical unit comprising a first light source and a first detector; aligning a second sample chamber with a second optical unit comprising a second light source and a second detector, wherein the first optical unit and the second optical unit are spatially separated from each other and the first optical unit and second optical unit are physically linked parts of an optical detection system; simultaneously performing (i) a first optical measurement of the first sample chamber using a first optical wavelength and (ii) a second optical measurement of a second sample chamber using a second optical wavelength, wherein simultaneously performing the first and second optical measurements includes; (a) simultaneously illuminating the first and second sample chambers using the first and second light sources, respectively, and (b) simultaneously receiving light from the first and second sample chambers using the first and second detectors, respectively; heating the first sample chamber with a first heater to create a first temperature progression at the first sample chamber; heating the second sample chamber with a second heater to create a second temperature progression at the second sample chamber; controlling the first and second heaters according to a PCR protocol to cause real time PCR reactions in the first and second sample chambers, and simultaneously quantifying a targeted and amplified DNA molecule by relative movement of the first optical unit and the second optical unit relative to the first and second sample chambers; and moving the first optical unit and the second optical unit relative to the first and second sample chambers so as to cause an alignment of the first sample chamber with the second optical unit and an alignment of the second sample chamber with the first optical unit. - View Dependent Claims (14)
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15. An optical multiplexing system for detecting sample components in at least two sample chambers, the system comprising:
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a first optical unit having a first light source and a first detector, said first optical unit detecting a product of a first polymerase chain reaction (PCR); a second optical unit having a second light source and a second detector, said second optical unit detecting a product of a second polymerase chain reaction (PCR), wherein the second optical unit is spatially separated from the first optical unit; a first heater associated with a first sample chamber of the at least two sample chambers, said first heater creating a first temperature progression at the first sample chamber; a second heater associated with a second sample chamber of the at least two sample chambers, said second heater creating a second temperature progression at the second sample chamber; a rotation frame having the first and second optical units fixed thereto; a motor adapted to rotate the rotation frame so as to cause the first and second optical units to move between a first position and a second position, wherein, when in the first position, the first optical unit is positioned with respect to a first sample chamber and the first heater so that the first light source illuminates and the first detector receives light from the first sample chamber, while the second optical unit is positioned with respect to a second sample chamber and the second heater so that the second light source illuminates and the second detector receives light from the second sample chamber, and said first and second optical units simultaneously illuminate the at least two sample chambers, and the first and second detectors simultaneously receive light from the at least two sample chambers, wherein said first and second optical units simultaneously perform first and second optical measurement using two different optical wavelengths at said at least two sample chambers; and a control unit for receiving a PCR protocol, wherein said control unit controls the first and second heaters according to the PCR protocol to cause real time PCR reactions in the first and second sample chambers, said control unit simultaneously quantifying a targeted and amplified DNA molecule by relative movement of the first optical unit and second optical unit relative to the at least two sample chambers. - View Dependent Claims (16, 17, 18)
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19. An optical multiplexing system for detecting sample components in at least two sample chambers, the system comprising:
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a first optical unit comprising a first light source and a first detector, said first optical unit detecting a product of a first polymerase chain reaction (PCR); a second optical unit comprising a second light source and a second detector, said second optical unit detecting a product of a second polymerase chain reaction (PCR), wherein the first and second optical units are spatially separated from each other, wherein the system receives at least two sample chambers to be received at positions corresponding to the first and second optical units so that the first and second light sources, respectively, simultaneously illuminate the at least two sample chambers, and the first and second detectors, respectively, simultaneously receive light from the at least two sample chambers, said first and second optical units simultaneously performing first and second optical measurements using two different optical wavelengths at said two spatially separated sample chambers; a first heater associated with a first sample chamber of the at least two sample chambers, said first heater creating a first temperature progression at the first sample chamber; a second heater associated with a second sample chamber of the at least two sample chambers, said second heater creating a second temperature progression at the second sample chamber; and a control unit for receiving a PCR protocol, wherein said control unit controls the first and second heaters according to the PCR protocol to cause real time PCR reactions in the first and second sample chambers, said control unit simultaneously quantifying a targeted and amplified DNA molecule by relative movement of the first optical unit and second optical unit relative to the at least two sample chambers, wherein the system is adapted for; performing a first optical measurement of the first sample chamber with the first optical unit, performing a second optical measurement of the second sample chamber with the second optical unit, and a relative movement of the first and second optical units relative to the at least two sample chambers in order to get to a second stationary position in which the first optical unit is aligned with the second sample chamber and the second optical unit is aligned with the first sample chamber.
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Specification