Method and device for immunoassay using nucleotide conjugates
First Claim
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1. A composition comprising:
- a FAB fragment disposed in a solubilizing agent and covalently linked to a first nucleotide; and
one or more signal elements each covalently linked to a second nucleotide,wherein;
said first nucleotide has one or more repeated sequences,the second nucleotide is bound to one of the one or more repeated sequences on said first nucleotide,the ratio of the FAB fragment to the signal element is controlled by the number of repeated sequences,the solubilizing agent is a matrix configured to be disposed within a conduit of a device configured to perform an immunoassay, anda 3′
-end or 5′
-end of the first nucleotide and the second nucleotide is protected from endogenous exonuclease activity from a blood sample within the conduit by incorporating a protective chemical group.
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Abstract
A composition of matter for use in an immunoassay devices and method comprising a signal antibody, e.g., FAB fragment, covalently linked to a first nucleotide; and one or more signal elements, e.g., signal enzymes such as ALP or fluorescent dyes, each covalently linked to a second nucleotide, wherein the first nucleotide has one or more repeated sequences, and the second nucleotide is bound to one of the one or more repeated sequences on said first nucleotide, and wherein the ratio of the signal antibody to the signal element is controlled by the number of repeated sequences.
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9 Claims
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1. A composition comprising:
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a FAB fragment disposed in a solubilizing agent and covalently linked to a first nucleotide; and one or more signal elements each covalently linked to a second nucleotide, wherein; said first nucleotide has one or more repeated sequences, the second nucleotide is bound to one of the one or more repeated sequences on said first nucleotide, the ratio of the FAB fragment to the signal element is controlled by the number of repeated sequences, the solubilizing agent is a matrix configured to be disposed within a conduit of a device configured to perform an immunoassay, and a 3′
-end or 5′
-end of the first nucleotide and the second nucleotide is protected from endogenous exonuclease activity from a blood sample within the conduit by incorporating a protective chemical group. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification