Nucleic acid analysis using sequence tokens
First Claim
1. A composition comprising a mixture of polynucleotides, wherein the polynucleotides in the mixture are made by combining a plurality of different polynucleotide samples, wherein:
- each of the different samples comprises non-overlapping segments of a genome, wherein each of the non-overlapping segments is attached to a sequence tag that identifies the sample from which that segment is derived; and
each of the sequence tags comprises two or more unique token sequences, wherein the token sequences are chosen from different sets of token sequences, and within each set, the token sequences are different from one another and are designed to not cross hybridize with one another or their complements.
5 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides methods and compositions for tagging nucleic acid sequence fragments, e.g., a set of nucleic acid sequence fragments from a single genome, with one or more unique members of a collection of oligonucleotide tags, or sequence tokens, which, in turn, can be identified using a variety of readout platforms. As a general rule, a given sequence token is used once and only once in any tag sequence. In addition, the present invention also provides methods for using the sequence tokens to efficiently determine variations in nucleotide sequences in the associated nucleic acid sequence fragments.
166 Citations
16 Claims
-
1. A composition comprising a mixture of polynucleotides, wherein the polynucleotides in the mixture are made by combining a plurality of different polynucleotide samples, wherein:
-
each of the different samples comprises non-overlapping segments of a genome, wherein each of the non-overlapping segments is attached to a sequence tag that identifies the sample from which that segment is derived; and each of the sequence tags comprises two or more unique token sequences, wherein the token sequences are chosen from different sets of token sequences, and within each set, the token sequences are different from one another and are designed to not cross hybridize with one another or their complements. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
-
Specification