Set of oligonucleotide probes as well as methods and uses thereto
First Claim
1. A set of oligonucleotide probes for detecting a genomic target of interest, the set comprising at least 100 oligonucleotide probes, each of the oligonucleotide probes having an identical length and comprising a different nucleotide sequence complementary to a different subsequence of the genomic target sequence of interest, each of the oligonucleotide probes further comprising a non-nucleotide unit and at least one label, wherein the non-nucleotide unit is positioned at a corresponding identical location of each of the oligonucleotide probes.
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Abstract
The present disclosure relates to a set of at least 100 single-stranded oligonucleotide probes directed against (or complementary to) portions of a genomic target sequence of interest. The present disclosure also relates to a method of detecting a genomic target sequence of interest using the set of oligonucleotide probes and a method of generating the set of oligonucleotide probes. Further, the present disclosure relates to a kit comprising the set of oligonucleotide probes and at least one further component.
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Citations
23 Claims
- 1. A set of oligonucleotide probes for detecting a genomic target of interest, the set comprising at least 100 oligonucleotide probes, each of the oligonucleotide probes having an identical length and comprising a different nucleotide sequence complementary to a different subsequence of the genomic target sequence of interest, each of the oligonucleotide probes further comprising a non-nucleotide unit and at least one label, wherein the non-nucleotide unit is positioned at a corresponding identical location of each of the oligonucleotide probes.
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15. A method of detecting a genomic target sequence of interest in a sample, said method comprising the steps of:
incubating a set of oligonucleotide probes with a sample comprising a genomic target sequence of interest, the incubating step being performed under conditions conducive to the binding of the set of oligonucleotide probes to the genomic target of interest; and
detecting hybridization of a plurality of the oligonucleotide probes of the set with the target sequence of interest in the sample, wherein the set of oligonucleotide probes comprises at least 100 oligonucleotide probes, each of the oligonucleotide probes having an identical length and having a different nucleotide sequence and being complementary to a different subsequence of the genomic target of interest, each of the oligonucleotide probes further comprising a non-nucleotide unit and at least one label, wherein the non-nucleotide unit is positioned at a corresponding identical location of each of the oligonucleotide probes and the at least one label is attached to the non-nucleotide unit.- View Dependent Claims (16, 17, 18, 19, 20, 21)
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22. A kit for the detection of a genomic target sequence of interest, the kit comprising:
a set of at least 100 oligonucleotide probes, each of the oligonucleotide probes having a non-nucleotide unit, at least one label, and an identical length, and each of the oligonucleotide probes further comprising a different nucleotide sequence complementary to a different subsequence of the genomic target sequence of interest, the non-nucleotide unit positioned at a corresponding identical location of each of the oligonucleotide probes, and the at least one label attached to the non-nucleotide unit, and a detection agent. - View Dependent Claims (23)
Specification