Rejuvenation or preservation of germ cells

• US 8,445,277 B2
• Filed: 06/24/2010
• Issued: 05/21/2013
• Est. Priority Date: 06/24/2010
• Status: Active Grant

• Current Assignee: Enterprise Science Fund, LLC (Intellectual Ventures LLC)
• Original Assignee: The Invention Science Fund I, L.L.C. (Intellectual Ventures LLC)
• Inventors: Hyde, Roderick A., Jung, Edward K. Y., Wood, Lowell L. Jr.
• Primary Examiner(s): Weber, Jon P
• Assistant Examiner(s): CONSTANTINE, CHARLES Z
• Application Number:
  US12/803,450
• Publication Number:
  US 20110318312A1
• Time in Patent Office: 1,062 Days
• Field of Search: None
• US Class Current: 435/374
• CPC Class Codes: A01N 1/0226 Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
• CPC Class Codes: A61K 35/54 Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
• CPC Class Codes: A61P 15/00 Drugs for genital or sexual disorders for disorders of sex hormones A61P5/24; Contraceptives

Abstract

Certain embodiments disclosed herein include, but are not limited to, at least one of compositions, methods, devices, systems, kits, or products regarding rejuvenation or preservation of germ cells or gametes. Certain embodiments disclosed herein include, but are not limited to, methods of modifying germ cells or gametes, or methods of administering modified germ cells or gametes to at least one biological tissue.

Claims

1. A modified reproductive cell, produced by the process of actively introducing at least one exogenous energy supplying factor into at least one intracellular compartment of a reproductive cell;

and

introducing at least one detection material including at least one of a radioactive, luminescent, colorimetric, fluorescent, odorous substance, sensor, or electronic identification device;

wherein the reproductive cell has not yet entered meiosis.

2. The modified reproductive cell of claim 1,

wherein the at least one exogenous energy supplying factor is at least one of exogenous creatine, exogenous phosphocreatine, exogenous chlorophyll, exogenous lactate, or exogenous glucose.

3. The modified reproductive cell of claim 1,

wherein the at least one exogenous energy supplying factor is exogenous ATP, exogenous ADP, exogenous AMP, or a precursor thereof.

4. The modified reproductive cell of claim 1, wherein the reproductive cell is located in situ, in vitro, in vivo, in utero, in planta, or ex vivo.

5. The modified reproductive cell of claim 1, wherein the reproductive cell is implantable or transplantable.

6. The modified reproductive cell of claim 1, wherein the reproductive cell is implantable or transplantable into a subject.

7. The modified reproductive cell of claim 6, wherein the subject is a plant, alga, or animal.

8. The modified reproductive cell of claim 6, wherein the subject is a vertebrate or invertebrate.

9. The modified reproductive cell of claim 6, wherein the subject is an amphibian, mammal, reptile, fish, or bird.

10. The modified reproductive cell of claim 6, wherein the subject is a human.

11. The modified reproductive cell of claim 1, wherein the reproductive cell is implantable or transplantable into a subject subsequent to modification.

12. A modified reproductive cell, produced by the process of actively introducing at least one exogenous energy supplying factor into at least one intracellular compartment of a reproductive cell;

and

introducing at least one detection material including at least one of a radioactive, luminescent, colorimetric, fluorescent, odorous substance, sensor, or electronic identification device;

wherein the reproductive cell has not yet completed mitosis.

13. The modified reproductive cell of claim 12, wherein the at least one exogenous energy supplying factor is at least one of exogenous creatine, exogenous phosphocreatine, exogenous chlorophyll, exogenous lactate, or exogenous glucose.

14. The modified reproductive cell of claim 12, wherein the at least one exogenous energy supplying factor is exogenous ATP, exogenous ADP, exogenous AMP, or a precursor thereof.

15. The modified reproductive cell of claim 12, wherein the reproductive cell is located in situ, in vitro, in vivo, in utero, in planta, or ex vivo.

16. The modified reproductive cell of claim 12, wherein the reproductive cell is implantable or transplantable into a subject.

17. The modified reproductive cell of claim 16, wherein the subject is a plant, alga, or animal.

18. The modified reproductive cell of claim 16, wherein the subject is a vertebrate or invertebrate.

19. The modified reproductive cell of claim 16, wherein the subject is an amphibian, mammal, reptile, fish, or bird.

20. The modified reproductive cell of claim 16, wherein the subject is a human.

22. The modified reproductive cell of claim 16, wherein the at least one exogenous energy supplying factor is at least one of exogenous creatine, exogenous phosphocreatine, exogenous chlorophyll, exogenous lactate, or exogenous glucose.

23. The modified reproductive cell of claim 16, wherein the at least one exogenous energy supplying factor is exogenous ATP, exogenous ADP, exogenous AMP, or a precursor thereof.

21. A modified reproductive cell, produced by the process of actively introducing at least one exogenous energy supplying factor into at least one intracellular compartment of a reproductive cell;

and

introducing at least one detection material including at least one of a radioactive, luminescent, colorimetric, fluorescent, odorous substance, sensor, or electronic identification device;

wherein the reproductive cell has not yet entered mitosis.

24. The modified reproductive cell of claim 21, wherein the reproductive cell is located in situ, in vitro, in vivo, in utero, in planta, or ex vivo.

25. The modified reproductive cell of claim 21, wherein the reproductive cell is implantable or transplantable into a subject.

26. The modified reproductive cell of claim 25, wherein the subject is a plant, alga, or animal.

27. The modified reproductive cell of claim 25, wherein the subject is a vertebrate or invertebrate.

28. The modified reproductive cell of claim 25, wherein the subject is an amphibian, mammal, reptile, fish, or bird.

29. The modified reproductive cell of claim 25, wherein the subject is a human.

30. A modified reproductive cell, produced by the process of actively introducing at least one exogenous energy supplying factor into at least one intracellular compartment of a reproductive cell;

and

introducing at least one detection material including at least one of a radioactive, luminescent, colorimetric, fluorescent, odorous substance, sensor, or electronic identification device;

wherein the reproductive cell has not yet completed phase I of meiosis.

31. The modified reproductive cell of claim 30, wherein the at least one exogenous energy supplying factor is at least one of exogenous creatine, exogenous phosphocreatine, exogenous chlorophyll, exogenous lactate, or exogenous glucose.

32. The modified reproductive cell of claim 30, wherein the at least one exogenous energy supplying factor is exogenous ATP, exogenous ADP, exogenous AMP, or a precursor thereof.

33. The modified reproductive cell of claim 30, wherein the reproductive cell is located in situ, in vitro, in vivo, in utero, in planta, or ex vivo.

34. The modified reproductive cell of claim 30, wherein the reproductive cell is implantable or transplantable into a subject.

35. The modified reproductive cell of claim 34, wherein the subject is a plant, alga, or animal.

36. The modified reproductive cell of claim 34, wherein the subject is a vertebrate or invertebrate.

37. The modified reproductive cell of claim 34, wherein the subject is an amphibian, mammal, reptile, fish, or bird.

38. The modified reproductive cell of claim 34, wherein the subject is a human.