Flow cell for a flow cytometer system

• US 8,445,286 B2
• Filed: 11/07/2007
• Issued: 05/21/2013
• Est. Priority Date: 11/07/2006
• Status: Active Grant

• Current Assignee: Accuri Cytometers, Inc. (Becton Dickinson & Co)
• Original Assignee: Accuri Cytometers, Inc. (Becton Dickinson & Co)
• Inventors: Bair, Nathaniel C., Rich, Collin A., Eadie, Mark Robert, Lehrmann, Rebecca Ann
• Primary Examiner(s): Turk, Neil N
• Application Number:
  US12/514,052
• Publication Number:
  US 20100118298A1
• Time in Patent Office: 2,022 Days
• Field of Search: None
• US Class Current: 436/52
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 2015/1413 Hydrodynamic focussing
• CPC Class Codes: Y10T 137/0352 Controlled by pressure
• CPC Class Codes: Y10T 137/0379 By fluid pressure
• CPC Class Codes: Y10T 137/0419 Fluid cleaning or flushing
• CPC Class Codes: Y10T 29/49826 Assembling or joining
• CPC Class Codes: Y10T 436/115831 Condition or time responsive
• CPC Class Codes: Y10T 436/117497 with a continuously flowing sample or carrier stream

Abstract

The flow cytometer system of the preferred embodiment includes a flow cell body that functions to contain, protect, and align the components of the flow cytometer system; a flow channel, coupled to the flow cell body, that functions to conduct and focus sample fluid through an interrogation zone; and a sample injection probe, removably coupled to the flow cell body, that functions to provide a uniform flow of sample fluid to the flow channel. The flow cytometer system is preferably designed for the flow cytometer field. The flow cytometer system, however, may be alternatively used in any suitable environment and for any suitable reason.

Claims

1. A flow cytometer system having an interrogation zone, the flow cytometer system comprising:

a flow cell body that is a unitary construction and that contains and aligns components of the flow cytometer system;

a focusing lens, coupled to and located within the flow cell body adjacent to the interrogation zone, configured to focus light that impinges the interrogation zone;

a flow channel, coupled to the flow cell body, that conducts and focuses sample fluid through the interrogation zone;

a sample injection probe, removably coupled to the flow cell body, that provides a sample fluid to the flow channel;

a sample injection probe body, removably coupled to the flow cell body, that contains the sample injection probe and aligns the sample injection probe with the flow channel;

a sample injection probe cleaning port, at least partially defined within the sample injection probe body and fluidically coupled to the sample injection probe, that is configured to relieve pressure from the sample injection probe and configured to clean the sample injection probe by introducing sheath fluid therein; and

a bubble purge port fluidically coupled to the flow cell body and defined in the flow cell body upstream from the interrogation zone for a flow direction from the sample injection probe to the interrogation zone, wherein the bubble purge port is configured to selectively purge bubbles prior to their entry into the flow channel.

2. The flow cytometer system of claim 1 wherein the flow cell body includes a receiving channel that receives and holds the flow channel and provides an opening through which the flow channel is radially exposed for an interrogation of the sample fluid within the flow channel.

3. The flow cytometer system of claim 1 wherein the flow channel is mounted within the flow cell body and is a small diameter capillary made from an optically clear material.

4. The flow cytometer system of claim 1 wherein the sample injection probe is a small diameter capillary made from a unitary piece of non-corrosive rigid material.

5. The flow cytometer system of claim 1 wherein the sample injection probe body includes a mating interface for mating to the flow cell body.

6. The flow cytometer system of claim 5 wherein the mating interface includes a conical geometry that is configured for alignment of the sample injection probe body with the flow cell body.

7. The flow cytometer system of claim 5 wherein the sample injection probe body defines a circumferential groove that holds an o-ring, wherein the o-ring creates a seal and removably couples the sample injection probe body with the flow cell body.

8. The flow cytometer system of claim 1 wherein the bubble purge port selectively clears debris prior to entry into the flow channel for the flow direction from the sample injection probe to the interrogation zone.

9. The flow cytometer system of claim 1 further comprising a hydrodynamic focusing region, connected to the flow channel, that passes sample fluids into the flow channel for interrogation, wherein the hydrodynamic focusing region includes a nozzle, having a substantially cylindrical body, that is adapted to receive a sample fluid and a sheath fluid.

10. The flow cytometer system of claim 1 wherein the sample injection probe cleaning port is further connected to the flow cell body.

11. The flow cytometer system of claim 1 further comprising a pressure monitoring tube, concentrically located about the sample injection probe in a substantially symmetrical manner, that monitors the pressure within the sample injection probe.

12. The flow cytometer system of claim 11 wherein the pressure monitoring tube is connected to a sample injection probe cleaning port, such that the recognition of pressure in the sample injection probe and release of the pressure in the sample injection probe through the sample injection probe cleaning port is substantially synchronized.

13. The flow cytometer system of claim 1 further comprising a sample vial and a sample vial mechanism, wherein the sample vial mechanism selectively holds and accurately aligns the sample vial with the sample injection probe.

14. The flow cytometer system of claim 13 further comprising a sample injection probe filter, connected at an upstream end of the sample injection probe and substantially adjacent to the sample vial, that substantially removes debris prior to entry into the sample injection probe and the flow channel, wherein the sample injection probe filter includes a porous medium having a predetermined pore size for filtering out particles having a selected diameter.

15. The flow cytometer system of claim 1 further comprising a base plate, wherein the flow cell body and the sample injection probe body are mounted to the base plate.

16. The flow cytometer system of claim 15 wherein the sample injection probe body further includes a threaded face and the base plate further includes a complimentary threaded receiver, wherein the threaded face and the threaded receiver are configured for mating of the sample injection probe body and the base plate.

17. The flow cytometer system of claim 1 further comprising a retaining ring, coupled to the flow cell body, that applies a substantially evenly distributed pressure around the diameter of the flow cell body.

18. The flow cytometer system of claim 1 wherein the bubble purge port is approximately orthogonal to the longitudinal axis of the flow channel.

19. A flow cytometer system having an interrogation zone, the flow cytometer system comprising:

a flow cell body that is a unitary construction and that contains and aligns components of the flow cytometer system;

a focusing lens, coupled to and located within the flow cell body adjacent to the interrogation zone, configured to focus light that impinges the interrogation zone;

a flow channel, coupled to the flow cell body, that conducts and focuses sample fluid through the interrogation zone;

a sample injection probe, removably coupled to the flow cell body, that provides a sample fluid to the flow channel;

a sample injection probe body, removably coupled to the flow cell body, that aligns the sample injection probe with the flow channel; and

a sample injection probe cleaning port, at least partially defined within the sample injection probe body and fluidically coupled to the sample injection probe, that is configured to relieve pressure from the sample injection probe and configured to clean the sample injection probe by introducing sheath fluid therein.