Zinc binding compounds and their method of use

• US 8,445,702 B2
• Filed: 02/18/2011
• Issued: 05/21/2013
• Est. Priority Date: 05/05/2003
• Status: Active Grant

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Gee, Kyle
• Primary Examiner(s): CHANDRAKUMAR, NIZAL S
• Application Number:
  US13/030,743
• Publication Number:
  US 20110159517A1
• Time in Patent Office: 823 Days
• Field of Search: 549/225, 436/84
• US Class Current: 549/225
• CPC Class Codes: C07D 311/82 Xanthenes
• CPC Class Codes: C08B 37/0039 Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/5308 for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
• CPC Class Codes: G01N 33/6872 Intracellular protein regulatory factors and their receptors, e.g. including ion channels

Abstract

The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of zinc ions in a sample. The metal chelating moiety of the zinc-binding compound is an analog of the well-known calcium chelator, BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid), wherein the chelating moiety has been modified from a tetraacetic acid moiety to a tri- di- or monoacetic moiety. This change in acetic acid groups on the metal chelating moiety results in the selective bindings of zinc ions in the presence of calcium ions, both of which are present in biological fluids and intracellular cytosolic fluid and organelles.

Claims

1. A compound having the formula:

2. The compound according to claim 1, wherein said R is H.

3. The compound according to claim 1, wherein said R is CH₂OC(O)CH₃.

4. The compound according to claim 1, wherein said compound is

5. The compound according to claim 1, wherein said compound is

6. A kit comprising at least one compound according to the formula:

7. The kit according to claim 6, wherein said kit independently further comprises;

a buffer, an additional detection reagent, a metal ion calibration reagent, a positive control, a metal ion indicator other than for zinc ions, an antibody or fragment thereof or a reference dye standard.

8. The kit according to claim 6, wherein said R is H.

9. The kit according to claim 6, wherein said R is CH₂OC(O)CH₃.

10. The kit according to claim 6, wherein said compound is