Method and apparatus for increasing adipose vascular fraction

• US 8,446,586 B2
• Filed: 10/13/2009
• Issued: 05/21/2013
• Est. Priority Date: 10/15/2008
• Status: Expired due to Fees

• Current Assignee: CosGenix, LLC
• Original Assignee: CosGenix, LLC
• Inventors: Wu, Allan Yang, Morrow, David Martin
• Primary Examiner(s): STOCK JR, GORDON J
• Application Number:
  US12/578,549
• Publication Number:
  US 20100112084A1
• Time in Patent Office: 1,316 Days
• Field of Search: None
• US Class Current: 356/432
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: C12N 5/0653 Adipocytes; Adipose tissue
• CPC Class Codes: C12N 5/0667 Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
• CPC Class Codes: G01N 21/31 Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry G01N21/72 takes precedence

Abstract

A method and device for processing mammalian adipose tissue such that the vascular rich fraction is separated from the vascular poor fraction, Mammalian adipose tissue in the form of morselated surgical biopsies and/or lipoaspirate from liposuction is placed within a novel syringe attached to a detection device measuring either color, light saturation, infra-red light, heme, iron or oxygen saturation. This process involves no label and minimal manipulation and handling of the tissue. This process and device may also be used intra-operatively under sterile conditions for immediate use within the same individual receiving liposuction or surgery.

Claims

1. A device that when a vascular rich fat layer has passed through a container containing a material including said vascular rich fat layer and a vascular poor fat layer comprising:

a) a means for applying pressure on said material in said container;

b) a detection chamber that contains said material urged out of said container;

c) a light source positioned at one side of said detection chamber outputting light of a selected wavelength;

said detection chamber being translucent or transparent to said selected wavelength;

d) a photodetector positioned which detects said light;

e) control electronics connected to said photodetector; and

an indicator connected to said electronics;

said vascular rich fat having a pure orange color, absorbing light at 590 nm and containing oxygenated hemoglobin, which absorbs more infrared light than red light, and deoxygenated hemoglobin, which absorbs more red light than infrared light;

said vascular poor fat having a pure yellow color and absorbing light at 570 nm.

2. A device as claimed in claim 1 in which said indicator is a lever.

3. A device as claimed in claim 1 in which said indicator is a light.

4. A device as claimed in claim 1 in which said selected wavelength corresponds to the pure yellow color of said vascular poor fat.

5. A device as claimed in claim 1 in which said selected wavelength corresponds to an absorption wavelength of iron in hemoglobin.

6. A device as claimed in claim 1 in which said selected wavelength corresponds to the pure orange color of said vascular rich fat.

8. A device as claimed in claim 1 in which said selected wavelength corresponds to an absorption wavelength of oxygenated hemoglobin,

9. A device as claimed in claim 1 in which said selected wavelength is in a range of 600 to 750 nm.

10. A device as claimed in claim 1 in which said selected wavelength corresponds to an absorption wavelength of deoxygenated hemoglobin,

11. A device as claimed in claim 1 in which said selected wavelength is in a range of 850 to 1000 nm.

12. A device as claimed in claim 1 in which said light source and said photodetector are positioned on a same side of said detection chamber.

13. A device as claimed in claim 1 in which said light source and said photodetector are positioned on opposite sides of said detection chamber.

14. A device as claimed in claim 1 in which said device is sterilizable.

15. A device as claimed in claim 1 in which said container is a syringe.

16. A device as claimed in claim 1 in which said means for applying pressure is a piston.

17. A device as claimed in claim 16 further comprising:

a) a motor connected to said control electronics positioned to push on said piston;

b) said control electronics is additionally programmed to turn off said motor when said material in said detection chamber absorbs at said selected wavelength.

18. A device as claimed in claim 1 in which said means for applying pressure is pressurized gas.

19. A device as claimed in claim 18 further comprising:

a) a solenoid valve connected to said control electronics and said means for applying pressure;

said control electronics is additionally programmed to activate said solenoid valve when said material in said detection chamber absorbs at said selected wavelength.

7. A device as claimed in claim in which said selected wavelength is in a range of 500 to 700 nm.