Wash ring assembly and method of use

• US 8,449,687 B2
• Filed: 02/13/2012
• Issued: 05/28/2013
• Est. Priority Date: 05/11/2007
• Status: Active Grant

• Current Assignee: Bio-Rad Laboratories Incorporated
• Original Assignee: Bio-Rad Laboratories Incorporated
• Inventors: Jafari, Nasser, Blecka, Lawrence, Tsai, Chris
• Primary Examiner(s): Barr, Michael
• Assistant Examiner(s): OSTERHOUT, BENJAMIN LEE
• Application Number:
  US13/372,300
• Publication Number:
  US 20120138099A1
• Time in Patent Office: 470 Days
• Field of Search: 134/18, 134/21, 134/22.11, 134/42, 134/44, 134/52, 134/56.R, 134/104.1, 134/104.2, 134/137, 134/140, 134/147, 134/148, 134/151, 134/152, 134/157, 134/902
• US Class Current: 134/22.11
• CPC Class Codes: B01L 13/02 for receptacle or instruments
• CPC Class Codes: B01L 3/021 Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
• CPC Class Codes: B08B 3/022 Cleaning travelling work B08B3/042 takes precedence
• CPC Class Codes: B08B 9/00 Cleaning hollow articles by methods or apparatus specially adapted thereto B08B6/00 takes precedence
• CPC Class Codes: G01N 35/1004 Cleaning sample transfer devices
• CPC Class Codes: Y10S 134/902 Semiconductor wafer

Abstract

Devices, including a wash ring assembly, and methods are provided for the removal of excess fluid or solids from the exterior or interior of a probe used to transfer fluids, for instance, in an automated assay device. Typically, a probe is used to aspirate and dispense a sample fluid material such as whole blood or a reagent. The devices and methods provided herein are useful for removing excess fluid from the exterior or interior of the probe so as to prevent dripping and cross-contamination between samples or reagents. It is also contemplated that, utilizing the devices and methods provided herein, washing and/or drying can be performed simultaneously as the probe is in motion, aspirating a sample and/or dispensing a sample.

Claims

1. A method for cleaning a probe in a robotic aspiration and dispensing system as the probe is in motion, aspirating and/or dispensing, the method comprising:

providing a robotic aspiration and dispensing system comprising a probe and a wash ring, the wash ring comprising;

a top surface,

a bottom surface,

an outer side surface,

an inner surface forming a bore comprising an inner diameter, a long axis, an upper portion with an opening in the top surface and a lower portion with an opening in the bottom surface,

an inlet comprising an opening in the top surface, bottom surface or outer side surface and an opening in the upper bore portion; and

an outlet comprising an opening in the top surface, bottom surface or outer side surface and an opening in the lower bore portion,

wherein the inner diameter of the bore is adapted to receive the probe such that when the probe is present in the bore, a space is formed between the exterior surface of the probe and the inner surface forming the bore, wherein the width of the space between the exterior surface of the probe and inner surface forming the bore is about 0.008 inches to about 0.12 inches,

wherein the diameter of the outlet opening in the lower bore portion is larger than the diameter of the inlet opening in the upper bore portion, and wherein the bore is cylindrical between the inlet opening in the upper bore portion and outlet opening in the lower bore portion;

wherein a portion of the probe to be washed is present in the bore forming the space between the probe and the inner surface of the bore,

adding fluid into the bore of the wash ring through the inlet;

removing fluid by suction from the bore of the wash ring through the outlet;

wherein the adding fluid and removing fluid steps can be performed as the probe is in motion, aspirating and/or dispensing.

2. The method according to claim 1 further comprising the following step:

guiding the vertical movement of the wash ring with a guide shaft along a longitudinal axis of the probe.

3. The method according to claim 1, wherein the inner surface of the bore is made of a hydrophobic material or coated with a hydrophobic material.

4. The method according to claim 3, wherein the hydrophobic material is plastic, silicon, polyethylene, or TEFLON.

5. The method according to claim 1, wherein the width of the space formed between the exterior surface of the probe and the inner surface forming the bore is about 0.01 inches to about 0.12 inches.

6. The method according to claim 1, wherein the inlet opening in the inner surface forming the bore and the outlet opening in the inner surface forming the bore are on opposing sides of the bore.

7. The method according to claim 1, wherein the ratio of the diameter of the outlet opening in the inner surface forming the bore to the diameter of the inlet opening in the inner surface forming the bore is about 2 to 1 or about 3 to 1.