Sensor instrument system including method for detecting analytes in fluids

• US 8,449,824 B2
• Filed: 07/02/2008
• Issued: 05/28/2013
• Est. Priority Date: 09/09/2002
• Status: Active Grant

• Current Assignee: Yizhong Sun
• Original Assignee: Yizhong Sun
• Inventors: Sun, Yizhong
• Primary Examiner(s): WHITE, DENNIS MICHAEL
• Application Number:
  US12/217,261
• Publication Number:
  US 20090261987A1
• Time in Patent Office: 1,791 Days
• Field of Search: 422/63, 422/68.1, 422/76, 422/82.01, 422/82.02, 422/82.03, 422/82.04, 422/88, 422/90, 422/98, 422/52, 422/73, 422/82.05, 422/82.08, 422/82.09, 422/82.11, 422/99, 422/102, 422/407, 422/501, 422/502, 422/503, 422/504, 436/149, 436/150, 436/151, 436/164, 436/177, 436/43, 436/63, 435/29, 435/4, 435/6, 435/7.1, 506/30, 250/214.1, 250/251, 250/576, 530/408, 714/752
• US Class Current: 422/82.01
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: G01N 2035/00831 identification of the sample, e.g. patient identity, place of sampling
• CPC Class Codes: G01N 27/126 comprising organic polymers
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 27/414 Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
• CPC Class Codes: G01N 33/0034 comprising neural networks or related mathematical techniques
• CPC Class Codes: G01N 33/0075 for multiple spatially distributed sensors, e.g. for environmental monitoring transmission systems for measured values G08C
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 35/00732 Identification of carriers, materials or components in automatic analysers
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A sensor instrument system for detecting and identifying analytes in fluids of a region contains a local sensor instrument and remote central station. The instrument includes a core technology employing a single sensor having two electrodes operated by an electrical frequency sweeping to generate two sets of patterned electrical information from a single measurement, a data transmission module and a GPS receiver module. The central station connects to a network means connected to a plurality of local receiving sites equipped with including the respective transceivers, so that the local analyte electrical information and geographic position information transmitted by the instrument can be wirelessly and remotely received and processed by the central station. The core technology further includes a reference sensor for eliminating background influence, a temperature programming to control adsorption and desorption of analytes, and usage of all kinds of adsorbent materials having chemical selectivities including the hydrogen selectivity to enhance detection and identification of analytes in fluids.

Claims

1. A sensor instrument system, comprising:

a. a central station remote to a sensor instrument, said instrument includes a first sensor electrically connected to a detection module, a transmission module and a global positioning system (GPS) receiver module;

b. said first sensor includes first and second electrodes that are electrically separated by a first nonconductive material, wherein said first sensor is brought into contact with a portion of a sample of a fluid containing an analyte to thereby provide a detectable information for said analyte in said fluid;

c. said detection module is configured to generate a multiplicity of frequencies of alternating current (AC) signals of a group which have a same magnitude and are applied to said first sensor, and thereby measure said detectable information based on said group of said applied frequencies, wherein said detectable information comprises two AC related electrical properties at each of said applied frequencies of said AC signals for said analyte in said fluid;

d. said GPS receiver module locates a geographic position of said analyte which is detected;

e. said transmission module is in electrical communication with said detection and GPS receiver modules for transmitting data of said detectable information and said located geographic position to said central station; and

f. said central station including means for storing said data and means for analyzing said detectable information in accordance with frequencies in a selected subgroup of said applied frequencies of said AC signals to thereby identify said analyte.

2. The system as claimed in claim 1, further comprising said AC related electrical properties include impedance, resistance, reactance, phase angle, voltage and current.

3. The system as claimed in claim 1, further comprising said first sensor serves as an analytical sensor and includes said first nonconductive material that is selected from the group consisting of polymer, polymer inorganic material composite, molecular sieves, particles of silica gel, alumina, porous carbon, calcium carbonate and fluorocarbon, liquid organic salts having organoammonium or organophosphonium cations coupled with nucleophilic anions or inorganic anions, composite of one of platinum group metals having ruthenium, rhodium, palladium, osmium, iridium, and platinum composited by filler consisting of inorganic or organic materials, polymer modified porous ceramic particles including porous alumina, or polymer modified porous silica particles, polymer modified fluorocarbon particles, polymer modified porous glass beads, and polymer coated solid support particles for preparation of a packed column.

4. The system as claimed in claim 3, said sensor instrument further comprising a second sensor having third and fourth electrodes that are electrically separated by a second nonconductive material identical to said first nonconductive material, said second sensor serves as a reference sensor which is identical to said analytical sensor for compensation of background influence including polymer film aging, humidity level and variations of electrical properties of said analytical sensor caused by temperature variations, wherein said reference sensor is electrically connected to said detection module.

5. The system as claimed in claim 4, further comprising said analytical sensor and reference sensor are disposable.

6. The system as claimed in claim 4, further comprising implementing means for temperature programming to said analytical and reference sensors.

7. The system as claimed in claim 4, wherein said first, second, third, and fourth electrodes are spatial electrodes, or are positioned onto the respective ceramic substrates, or are positioned onto the respective silicon substrates.

8. The system as claimed in claim 7, further comprising surfaces of the respective solid support particles, ceramic and silicon substrates are treated with a surface treatment reagent including dimethyldichlorosilane, hexamethyldisilazane, trimethylchlorosilane, octadecyldimethylchlorosilane, or any combination thereof.

9. The system as claimed in claim 4, further comprising means for electromagnetically shielding to said analytical sensor and reference sensor.

10. The system as claimed in claim 4, further comprising a third nonconductive material is identical to said first nonconductive material and is located in front of said reference sensor, the remaining portion of said sample of said fluid containing said analyte separately flows towards said reference sensor to thereby come into contact with said third nonconductive material, so that said analyte is retained and said fluid is not retained by said third nonconductive material which results in only said fluid without having said analyte that is brought into contact with said reference sensor, said reference sensor is applied with said multiplicity of said frequencies of said AC signals of said group to thereby provide a detectable information for said fluid, wherein said detectable information comprises two AC related electrical properties at each of said applied frequencies of said AC signals for said fluid.

11. The system as claimed in claim 10, further comprising means for combining said electrical properties for said analyte in said fluid with said electrical properties for said fluid without having said analyte to obtain combined electrical properties of said analyte, and said combined electrical properties are capable of identifying said analyte in accordance with frequencies in a selected subgroup of said applied frequencies of said AC signals.

12. The system as claimed in claim 10, further comprising said analyte in said fluid flows through said analytical sensor and said fluid without having said analyte flows through said reference sensors in a laminar flow fashion.

13. The system as claimed in claim 1, further comprising said sensor instrument comprises a third sensor which is selected from the group consisting of a metal oxide thin film resistor sensor, conductive polymer sensor, polymer coated quartz crystal microbalance sensor, polymer coated surface acoustic wave sensor, metal-oxide-silicon field-effect-transistor sensor, dye coated optical fiber sensor, dye-impregnated bread array, micromachined cantilver array, polymer carbon black chemiresistor sensor, electrically conductive sensor comprising alternating regions of a conductive material and a material compositionally different than the conductive material between two conductive leads wherein said sensor provides an electrical path through the regions of conductive material and the regions of the compositionally different material, and any combination thereof.

14. The system as claimed in claim 1, further comprising a local receiving site having means for electrically communicating with said instrument, said local receiving site is electrically connected to said central station through connection of a network means comprising an Ethernet, an intranet, the internet, and the Internet linked by fiber-optical cables, metal wires, and wireless connection including electromagnetic waves.

15. The system as claimed in claim 1, further comprising said detection module including an analog to digital converter.

16. The system as claimed in claim 1, further comprising said sample is an environmental sample, which is selected from the group consisting of an environmental air sample, a headspace of an environmental liquid, and a headspace of an environmental solid.

17. The system as claimed in claim 1, further comprising said sample is a biological sample, which is selected from the group consisting of a body odor sample, a breath sample, headspaces of the respective urine sample, blood sample, stool sample, serum sample and saliva sample.

18. The system as claimed in claim 1, further comprising said sample is an anaesthetic sample.

19. The system as claimed in claim 1, further comprising said sample has an identity.

20. The system as claimed in claim 1, further comprising said central station includes means for comparing said detectable information in accordance with said frequencies in said selected subgroup for said analyte with information of the respective known analytes which is prestored in a database of said central station.

21. The system as claimed in claim 20, further comprising said central station includes means for comparing a pattern of said detectable information in accordance with said frequencies in said selected subgroup for said analyte with patterns of said information of the respective known analytes which are prestored in said database of said central station.

22. The system as claimed in claim 21, further comprising a pattern recognition algorithm of principal component analysis capable of identifying said pattern of said detectable information for said analyte.

23. The system as claimed in claim 22, further comprising additional analysis algorithms respectively capable of analyzing said detectable information for said analyte, wherein said additional analysis algorithms comprising soft independent modeling of class analogy, k nearest neighbor, hierarchical cluster analysis, canonical discriminant analysis, classical least squares, principal component regression, partial least squares regression, supervised and unsupervised learning neural network, fuzzy neural network techniques, and combination thereof.

24. The system as claimed in claim 1, further comprising said analyte is selected from the group consisting of studies of materials containing illegal substances, environment concerns, medical interests including hospital concerns, scientific and research interests including space research interests, interests of industrial sectors including food, beverages, agricultural, chemical, petroleum, plastic, construction, pharmaceutical, automobile, biochemical, and transportation, consumer interests including perfume, cosmetic, wine and flavor, and safety concerns including explosive, arson and road spill investigation.

25. The system as claimed in claim 1, further comprising said frequencies of said AC signals are selected from the group consisting of a sinusoidal wave having swept frequencies and a multiplicity of sinusoidal waves having the respective different frequencies.

26. The system as claimed in claim 1, further comprising said sensor instrument includes sample handling, heating and power modules that are respectively and electrically connected to a microcomputer module that is electrically connected to said detection, transmission and GPS receiver modules, wherein said heating module includes an exterior heater attached to an exterior surface of a sensor compartment of a sensor compartment module of said sensor instrument.

27. The system as claimed in claim 1, further comprising said sensor instrument providing selection of starting frequency, stopping frequency and said magnitude of said AC signals.

28. A sensor instrument system, comprising:

a sensor instrument, said instrument comprises a dual sensor electrically connected to a detection module that is in electrical communication with a transmission module, wherein;

a. said dual sensor including a first sensor serving as an analytical sensor and a second sensor serving as a reference sensor identical to said analytical sensor, wherein said analytical sensor comprises first and second electrodes that are electrically separated by a first nonconductive material, said reference sensor comprises third and fourth electrodes that are electrically separated by a second nonconductive material which is identical to said first nonconductive material, said analytical sensor is brought into contact with a first fluid containing an analyte, said reference sensor is brought into contact with a second fluid that is identical to said first fluid but does not contain said analyte;

b. said detection module is configured to generate a multiplicity of frequencies of alternating current (AC) signals of a group which have a same magnitude and are applied to said analytical sensor contacted with said first fluid containing said analyte, and thereby measure a detectable information for said analytical sensor based on said group of said applied frequencies wherein said detectable information comprises two AC related electrical properties at each of said applied frequencies, said detection module is configured to generate said multiplicity of said frequencies of said alternating current (AC) signals of said group which have said same magnitude and are applied to said reference sensor contacted with said second fluid, and thereby measure a detectable information for said reference sensor based on said group of said applied frequencies wherein said detectable information comprises two AC related electrical properties at each of said applied frequencies, combined AC related electrical properties are obtained for said analyte by combining said AC related electrical properties of said analytical sensor with the respective AC related electrical properties of said reference sensor, said combined AC related electrical properties are capable of identifying said analyte according to frequencies in a selected subgroup of said applied frequencies; and

c. said transmission module transmits data of said identified analyte.

29. The system as claimed in claim 28, further comprising said first nonconductive material is selected from the group consisting of polymer, polymer inorganic material composite, molecular sieves, particles of silica gel, alumina, porous carbon, calcium carbonate and fluorocarbon, liquid organic salts having organoammonium or organophosphonium cations coupled with nucleophilic anions or inorganic anions, composite of one of platinum group metals having ruthenium, rhodium, palladium, osmium, iridium, and platinum composited by filler consisting of inorganic or organic materials, polymer modified porous ceramic particles including porous alumina, or polymer modified porous silica particles, polymer modified fluorocarbon particles and polymer modified porous glass beads, and polymer coated solid support particles for preparation of a packed column.

30. The system as claimed in claim 28, further comprising a central station in electrical communication with said sensor instrument wherein said central station is capable of storing and displaying said data of said identified analyte.

31. The system as claimed in claim 28, further comprising said analyte is selected from the group consisting of studies of materials containing illegal substances, environment concerns, medical interests including hospital concerns, scientific and research interests including space research interests, interests of industrial sectors including food, beverages, agricultural, chemical, petroleum, plastic, construction, pharmaceutical, automobile, biochemical, and transportation, consumer interests including perfume, cosmetic, wine and flavor, and safety concerns including explosive, arson and road spill investigation.

32. A sensor instrument system, comprising:

a sensor instrument, said instrument including a sensor electrically connected to a detection module that is in electrical communication with a transmission module, wherein;

a. said sensor comprises first and second electrodes that are electrically separated by an air gap, said sensor is brought into contact with a fluid containing an analyte;

b. said detection module is configured to generate a multiplicity of frequencies of alternating current (AC) signals of a group which have a same magnitude and are applied to said sensor contacted with said fluid containing said analyte, and thereby measure a detectable information of said sensor based on said group of said applied frequencies, wherein said detectable information comprises two AC related electrical properties at each of said applied frequencies, said AC related electrical properties are capable of identifying said analyte according to frequencies in a selected subgroup of said applied frequencies of said AC signals; and

c. said transmission module transmits data of said identified analyte.

33. The system as claimed in claim 32, further comprising a central station in electrical communication with said sensor instrument wherein said central station is capable of storing and displaying said data of said identified analyte.