System for management of yeast to facilitate the production of ethanol

• US 8,450,094 B1
• Filed: 03/03/2010
• Issued: 05/28/2013
• Est. Priority Date: 03/03/2009
• Status: Active Grant

• Current Assignee: Poet Research Inc.
• Original Assignee: Poet Research Inc.
• Inventors: Narendranath, Neelakantam V., Carlson, David Charles
• Primary Examiner(s): Martinell, James
• Application Number:
  US12/717,002
• Time in Patent Office: 1,182 Days
• Field of Search: None
• US Class Current: 435/161
• CPC Class Codes: C12M 25/00 Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
• CPC Class Codes: C12M 35/04 Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
• CPC Class Codes: C12M 41/12 of temperature controlling the temperature of chemical or physical processes B01J19/0013, heating or cooling apparatus for laboratory use B01L7/00
• CPC Class Codes: C12N 1/16 Yeasts; Culture media therefor
• CPC Class Codes: C12N 1/18 Baker's yeast; Brewer's yeast
• CPC Class Codes: C12N 1/22 Processes using, or culture media containing, cellulose or hydrolysates thereof
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

A system and method for managing an ethanologen for use in biorefinery is disclosed. The method for propagating ethanologen for use in the production of a fermentation product from biomass omprises the steps of providing a medium for propagation of ethanologen and supplying a first cell mass of ethanologen to the medium. A first cell mass of ethanologen is propagated into a larger second cell mass of ethanologen. The system for propagating ethanologen for use in the production of a fermentation product from biomass comprises a first stage comprising a first vessel configured to maintain a medium comprising ethanologen and a second stage comprising a second vessel configured to maintain a medium supplied from the first stage.

Claims

1. A method of propagating ethanologen for use in the production of ethanol from biomass comprising the steps of:

providing a medium for propagation of ethanologen;

supplying a first cell mass of ethanologen to the medium;

supplying xylose to the medium as a carbon source for cell mass growth of the ethanologen;

maintaining the medium comprising the first cell mass of ethanologen at a pH of between about 5.0 and 6.0 and at a temperature of between about 26 and about 37 degrees Celsius so that the first cell mass of ethanologen is propagated into a second cell mass of ethanologen, under aerobic conditions with an airflow of at least 1.0 volumes of air per volume of medium per minute;

and wherein the second cell mass of ethanologen is larger than the first cell mass of ethanologen.

2. The method of claim 1 wherein xylose is the sole source of carbon supplied to the medium.

3. The method of claim 2 wherein the ethanologen is derived from Saccharomyces cerevisiae.

4. The method of claim 2 wherein the ethanologen comprises yeast, and the second cell mass of ethanologen comprises about 0.35 to 0.55 grams dry yeast per gram of sugar.

5. The method of claim 2 wherein xylose is supplied to the medium at a concentration of at least 1 percent by weight of medium.

6. The method of claim 2 wherein xylose is supplied to the medium at a concentration of at least 3 percent by weight of medium.

7. The method of claim 2 wherein xylose is supplied to the medium at a concentration of about 6 percent by weight of medium.

8. The method of claim 2 wherein the step of providing a first medium for propagation of the ethanologen comprises a first stage and further comprising the steps of:

providing a second medium for propagation of ethanologen in a second stage;

supplying the second cell mass of ethanologen to the second medium;

maintaining the second medium comprising the second cell mass of ethanologen at a pH of between about 5.0 and 6.0 and at a temperature of between about 26 and about 37 degrees Celsius so that the second cell mass of ethanologen is propagated into a third cell mass of ethanologen;

wherein the third cell mass of ethanologen has a cell mass that is larger than the cell mass of the second cell mass of ethanologen.

9. The method of claim 8 wherein xylose is provided to the second medium as a carbon source for the ethanologen in the second stage.

10. The method of claim 8 wherein the third cell mass is at least 20 times larger than the second cell mass.

11. The method of claim 8 wherein the second cell mass is at least 200 times larger than the first cell mass.

12. The method of claim 11 wherein the propagation of the first cell mass of ethanologen into the second cell mass of ethanologen and the propagation of the second ethanologen into the third cell mass of ethanologen comprise a total time of no more than about 80 hours.

13. The method of claim 1 wherein the biomass comprises lignocellulosic material.

14. The method of claim 13 wherein the lignocellulosic material comprises at least one of corn cobs, corn plant husks, corn plant leaves and corn plant stalks.

15. The method of claim 13 wherein the xylose supplied to the medium is obtained from the lignocellulosic material.

16. The method of claim 15 wherein xylose is obtained as a component of the biomass and wherein xylose is supplied to the medium comprises at a rate of at least 0.12 grams of xylose per minute.

17. The method of claim 16 wherein the component comprises xylose at about 2 to 5 percent by weight.

18. The method of claim 16 wherein the component comprises xylose and glucose and the ethanologen comprises an organism capable of fermenting xylose into ethanol and glucose into ethanol.

19. The method of claim 18 wherein the ethanologen comprises Saccharomyces cerevisiae.

20. The method of claim 1 wherein the ethanologen comprises yeast cells capable of fermenting pentose into ethanol.

21. The method of claim 1 wherein the ethanologen comprises yeast cells capable of fermenting xylose into ethanol.

22. The method of claim 1 wherein xylose has been used as a carbon source for growth of the second cell mass of ethanologen.

23. The method of claim 22 wherein the use of xylose for growth of the first cell mass into the second cell mass of ethanologen is such that at least 80 percent of the xylose is consumed.

24. The method of claim 1 wherein the second cell mass of ethanologen comprises an inoculum of the ethanologen that can be provided to a fermentation system for fermentation of pentose.

25. The method of claim 1 further comprising the step of providing an agent to the medium;

wherein the agent comprises at least one nutrient.

26. The method of claim 25 wherein the agent comprises at least one of yeast extract, urea, diammonium phosphate, magnesium sulfate or zinc sulfate.

27. The method of claim 1 wherein the ethanologen comprises a strain of Saccharomyces cerevisiae capable of converting xylose into ethanol.

28. The method of claim 1 wherein the step of maintaining the medium comprises applying xylose to the medium and maintaining the medium at about 30 degrees Celsius for about 120 hours.

29. The method of claim 1 wherein the step of maintaining the medium comprises aerating a yeast inoculum.

30. The method of claim 1 wherein the biomass comprises hemicellulose and the fermentation product has been produced by fermentation of xylose.

31. The method of claim 1 wherein the biomass comprises cellulose and the fermentation product has been produced by fermentation of glucose.

32. A method of propagating ethanologen for use in the production of ethanol from biomass comprising the steps of:

providing a medium for propagation of ethanologen;

supplying a first cell mass of ethanologen to the medium;

providing an agent to the medium;

providing a component obtained from the biomass to the medium as a carbon source for the ethanologen;

maintaining the medium comprising the first cell mass of ethanologen at a pH of between about 5.0 and 6.0 and at a temperature of between about 26 and about 37 degrees Celsius so that the first cell mass of ethanologen is propagated into a second cell mass of ethanologen, wherein the medium comprising the ethanologen is aerated with an airflow of at least 1.0 volumes of air per volume of medium per minute;

wherein the second cell mass of ethanologen is larger than the first cell mass of ethanologen;

wherein the biomass comprises lignocellulosic material;

wherein the lignocellulosic material comprises at least one of corn cobs, corn plant husks, corn plant leaves and corn plant stalks;

wherein the component is obtained from the lignocellulosic material;

wherein the component comprises pentose;

wherein pentose comprises the carbon source for the ethanologen; and

wherein the ethanologen comprises yeast cells capable of fermenting pentose into ethanol.

33. The method of claim 32 wherein the second cell mass of ethanologen comprises an inoculum of the ethanologen that can be provided to a fermentation system for fermentation of pentose.

34. The method of claim 32 wherein pentose comprises xylose.

35. The method of claim 34 wherein the component comprises xylose at about 2 to 5 percent by weight.

36. The method of claim 34 wherein the biomass comprises hemicellulose and the fermentation product has been produced by fermentation of xylose.

37. The method of claim 34 wherein the biomass comprises hemicellulose and the fermentation product has been produced by fermentation of glucose.

38. The method of claim 32 wherein the ethanologen comprises a strain of Saccharomyces cerevisiae capable of converting xylose into ethanol.

39. The method of claim 32 wherein the step of maintaining the medium comprises applying the component to the medium and maintaining the medium at about 30 degrees Celsius for about 120 hours.

40. The method of claim 39 wherein the medium comprises sugar, the ethanologen comprises yeast and the second cell mass of ethanologen comprises about 0.35 to 0.55 grams dry yeast per gram of sugar.

41. The method of claim 32 wherein the agent comprises at least one of yeast extract, urea, diammonium phosphate, magnesium sulfate, zinc sulfate.

42. The method of claim 32 wherein the step of supplying the component to the medium comprises xylose supplied at a rate of at least 0.12 grams of xylose per minute.

43. The method of claim 32 wherein the component comprises xylose and glucose and the ethanologen comprises an organism capable of fermenting xylose into ethanol and glucose into ethanol.

44. The method of claim 43 wherein the ethanologen comprises Saccharomyces cerevisiae.

45. The method of claim 32 further comprising the steps of:

providing a second medium for propagation of ethanologen;

supplying the second cell mass of ethanologen to the second medium;

maintaining the second medium comprising the second cell mass of ethanologen at a pH of between about 5.0 and 6.0 and at a temperature of between about 26 and about 37 degrees Celsius so that the second cell mass of ethanologen is propagated into a third cell mass of ethanologen;

wherein the third cell mass of ethanologen has a cell mass that is larger than the cell mass of the second cell mass of ethanologen.

46. The method of claim 45 wherein the propagation of the first cell mass of ethanologen into the second cell mass of ethanologen and the propagation of the second ethanologen into the third cell mass of ethanologen comprise a total time of no more than about 80 hours.

47. A system for propagating ethanologen for use in the production of ethanol from biomass in a fermentation system comprising:

a first stage comprising a first vessel configured to maintain a medium comprising ethanologen;

a second stage comprising a second vessel configured to maintain a medium supplied from the first stage;

a source of xylose to be provided to the medium as a carbon source for ethanologen in the first stage;

a source of xylose to be provided to the medium as a carbon source for the ethanologen in the second stage;

wherein the ethanologen has a first cell mass when supplied to the first stage and the ethanologen has a second cell mass when supplied from the first stage to the second stage and the ethanologen has a third cell mass when supplied from the second stage;

wherein the medium in the first vessel is maintained at a pH of between about 5.0 and 6.0 and at a temperature of between about 26 and about 37 degrees Celsius with an airflow of at least 1.0 volumes of air per volume of medium per minute so that ethanologen can be propagated into the second cell mass;

wherein the medium in the second vessel is maintained at a pH of between about 5.0 and 6.0 and at a temperature of between about 26 and about 37 degrees Celsius with an airflow of at least 1.0 volumes of air per volume of medium per minute so that ethanologen can be propagated into the third cell mass;

wherein the second cell mass is at least 200 times larger than the first cell mass;

wherein the third cell mass is at least 20 times larger than the second cell mass.

48. The system of claim 47 further comprising an apparatus to pre-treat the biomass into a liquid component comprising the source of xylose for the ethanologen.

49. A method of propagating ethanologen for use in the production of ethanol from biomass comprising the steps of:

providing a medium for propagation of ethanologen;

supplying a first cell mass of ethanologen having a first cell mass to the medium;

supplying xylose to the medium as a carbon source for the ethanologen;

maintaining the medium comprising the first cell mass of ethanologen at a pH of between about 5.0 and 6.0 and at a temperature of between about 26 and about 37 degrees Celsius under aerobic conditions so that the first cell mass of ethanologen is propagated into a second cell mass of ethanologen, under aerobic conditions comprises a supply of air of at least 1.0 liters per liter of medium per minute so that xylose is used for cell growth instead of production of ethanol;

and wherein the second cell mass of ethanologen is larger than the first cell mass of ethanologen.

50. The method of claim 49 wherein xylose is the sole source of carbon supplied to the medium.

51. The method of claim 49 wherein xylose is supplied to the medium at a concentration of at least 3 percent (by weight in medium).

52. The method of claim 49 wherein xylose is supplied to the medium at a concentration of about 6 percent (by weight in medium).

53. The method of claim 49 wherein the biomass comprises lignocellulosic material.