Peptide-based scaffolds for cartilage regeneration and methods for their use

• US 8,450,271 B2
• Filed: 04/13/2010
• Issued: 05/28/2013
• Est. Priority Date: 04/13/2009
• Status: Active Grant

• Current Assignee: Northwestern University
• Original Assignee: Northwestern University
• Inventors: Shah, Ramille N., Shah, Nirav A., Stupp, Samuel I.
• Primary Examiner(s): Cordero Garcia, Marcela M
• Application Number:
  US12/759,683
• Publication Number:
  US 20100266557A1
• Time in Patent Office: 1,141 Days
• Field of Search: None
• US Class Current: 514/8.9
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 38/1841 Transforming growth factor [TGF]
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/02 for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: C07K 14/00 Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
• CPC Class Codes: C07K 7/06 having 5 to 11 amino acids

Abstract

Disclosed herein are novel peptide amphiphile molecules and compositions composed of a peptide sequence that non-covalently binds the growth factor TGF-β1. Also disclosed are methods of using these peptide amphiphiles to create a gel scaffold in situ that enhances articular cartilage regeneration when used in combination with microfracture. Significant improvement in tissue quality and overall O'"'"'Driscoll histological scores were observed in rabbits with full thickness articular cartilage defects treated with the TGF-binding peptide amphiphile. The gel can further serve as a delivery vehicle for recombinant TGF-β1 protein growth factor. Scaffolds that localize and retain chondrogenic growth factors may synergistically enhance cartilage repair when combined with microfracture, by inducing bone marrow mesenchymal stem cells into chondrogenic differentiation. This invention represents a promising new biomimetic approach to enhance current techniques of articular cartilage regeneration in the clinical setting.

Claims

1. A synthetic peptide comprising the sequence HSNGLPLGGGSEEEAAAVVVK (SEQ ID NO:

3).

18. A method of treating an articular cartilage defect or lesion in a patient in need thereof, comprising debridement of a cartilage defect or lesion on the articular surface of a joint in a patient in need thereof, and contacting the debrided surface of the joint with the composition of claim 1.

19. The method of claim 18, wherein the composition is administered as an adjuvant to an orthopedic surgical procedure intended to repair, restore or regenerate damaged or missing cartilage and wherein said orthopedic surgical procedure is an open two-state autologous chondrocyte implantation (ACI), wherein said composition is combined with the autologous chondrocytes and forms a gel scaffold containing the cells in lesion site.

20. The method of claim 18, wherein the composition is administered as an adjuvant to an orthopedic surgical procedure intended to repair, restore or regenerate damaged or missing cartilage and wherein said orthopedic surgical procedure is an open osteochondral allograft transplantation (OATS), wherein said composition forms a gel in the lesion site.

22. A kit for the in vitro formation of self-assembled micelles for administration into a patient comprising a peptide amphiphile of claim 1 and one or more aqueous components, wherein said one or more aqueous components are used to dissolve said peptide amphiphiles sufficient to induce self-assembly or gel formation, said self-assembly resulting from changes in the pH of the solution or the presence of multivalent ions, charged polymers or other charged macromolecules in said components.

23. The kit of claim 22, optionally comprising components suitable to remove cells in the patient for incorporation into the formulation for administration to the patient.

2. A peptide amphiphile consisting of the following segments:

(1) a growth-factor-binding peptide segment selected from among SEQ ID NOs;

21-40;

(2) a spacer segment comprising GGGSEEE (SEQ ID NO;

5);

(3) a beta-sheet forming, structural peptide segment, and (4) a non-peptide lipophilic segment.

3. The peptide amphiphile of claim 2 wherein the structural peptide segment is AAAVVVK (SEQ ID NO:

10).

4. The peptide amphiphile of claim 2 wherein the lipophilic segment is comprised of a single, saturated, linear alkyl chain of the formula:

CnH2n-1O-, where n 6-22, and wherein the lipophilic segment is covalently linked to the epsilon amine of a C-terminal lysine residue.

6. A composition comprising one or more peptide amphiphiles selected from the compounds of claim 2, mixed with one or more filler peptide amphiphiles and dispersed in an aqueous medium, wherein said filler peptide amphiphile comprises the sequence VVVAAAEEE (SEQ ID NO:

7).

7. The composition of claim 6 wherein said peptide amphiphile composition further comprises one or more recombinant human proteins of the transforming growth factor (TGF) superfamily.

8. The composition of claim 7 wherein said recombinant human protein is rhTGF-β

1.

9. A method of treating an articular cartilage defect or lesion in a patient in need thereof said method comprising debridement of a cartilage defect or lesion on articular surface of a joint in a patient in need thereof, and contacting the debrided surface of the joint with the composition claim 6 to said patient.

10. The method of claim 9 wherein said composition is administered as an adjuvant to an orthopedic surgical procedure intended to repair, restore or regenerate damaged or missing cartilage.

11. The method of claim 9 wherein said surgical procedure is microfracture, and said composition forms a gel-clot with autologous blood and cells released from the osteochondral bone or marrow during the microfracture procedure.

12. The method of claim 9 wherein the surgical procedure is an open two-stage autologous chondrocyte implantation (ACI), wherein said composition is combined with the autologous chondrocytes and forms a gel scaffold containing the cells in the lesion site.

13. The method of claim 9 wherein the surgical procedure is an open osteochondral allograft transplantation (OATS), wherein said composition forms a gel in the lesion site.

14. The method of claim 9 wherein said composition is administered arthroscopically.

15. The method of claim 9, wherein the patient is human.

16. The method of claim 9, wherein the patient is an animal.

17. The method of claim 9, wherein the patient is a horse, dog, sheep, goat, or cow.

21. A substrate coated with self-assembled micelles formed by the peptide amphiphile composition of claim 2.

24. A kit for making an injectable formulation for the in vivo formation of cylindrical micelles in a patient in need thereof comprising a peptide amphiphile composition of claim 2.

25. The kit of claim 24, optionally comprising components suitable to remove cells in the patient for incorporation into the formulation for administration to the patient.

26. A method of concentrating and protecting endogenous TGF-β

1 released by cells in an area of articular cartilage deficiency comprising the step of administering to a patient in need thereof a composition of claim 6, wherein the administration step is performed during a microfracture procedure.

27. A method of concentrating and protecting endogenous TGF-β

1 released by cells in an area of articular cartilage deficiency comprising the step of administering to a patient in need thereof a composition of claim 6, wherein the administration step is performed during an ACI procedure.

5. A peptide amphiphile consisting of the following structure (SEQ ID NO:

4).