Peptide-based scaffolds for cartilage regeneration and methods for their use
First Claim
1. A synthetic peptide comprising the sequence HSNGLPLGGGSEEEAAAVVVK (SEQ ID NO:
- 3).
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Abstract
Disclosed herein are novel peptide amphiphile molecules and compositions composed of a peptide sequence that non-covalently binds the growth factor TGF-β1. Also disclosed are methods of using these peptide amphiphiles to create a gel scaffold in situ that enhances articular cartilage regeneration when used in combination with microfracture. Significant improvement in tissue quality and overall O'"'"'Driscoll histological scores were observed in rabbits with full thickness articular cartilage defects treated with the TGF-binding peptide amphiphile. The gel can further serve as a delivery vehicle for recombinant TGF-β1 protein growth factor. Scaffolds that localize and retain chondrogenic growth factors may synergistically enhance cartilage repair when combined with microfracture, by inducing bone marrow mesenchymal stem cells into chondrogenic differentiation. This invention represents a promising new biomimetic approach to enhance current techniques of articular cartilage regeneration in the clinical setting.
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Citations
27 Claims
- 1. A synthetic peptide comprising the sequence HSNGLPLGGGSEEEAAAVVVK (SEQ ID NO:
-
2. A peptide amphiphile consisting of the following segments:
- (1) a growth-factor-binding peptide segment selected from among SEQ ID NOs;
21-40;
(2) a spacer segment comprising GGGSEEE (SEQ ID NO;
5);
(3) a beta-sheet forming, structural peptide segment, and (4) a non-peptide lipophilic segment. - View Dependent Claims (3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 21, 24, 25, 26, 27)
- (1) a growth-factor-binding peptide segment selected from among SEQ ID NOs;
-
5. A peptide amphiphile consisting of the following structure (SEQ ID NO:
- 4).
Specification