Method for sorting resistant seed from a mixture with susceptible seed

• US 8,459,463 B2
• Filed: 12/21/2010
• Issued: 06/11/2013
• Est. Priority Date: 04/24/2007
• Status: Expired due to Fees

• Current Assignee: Pioneer Hi-Bred International (Corteva, Inc.)
• Original Assignee: Pioneer Hi-Bred International (Corteva, Inc.)
• Inventors: Hunter, James L., Mangold, Gregory K.
• Primary Examiner(s): MATTHEWS, TERRELL HOWARD
• Application Number:
  US12/974,513
• Publication Number:
  US 20110143936A1
• Time in Patent Office: 903 Days
• Field of Search: 209/3, 209/3.3, 209/47, 209/49, 209/51, 209/552, 209/936, 209/938
• US Class Current: 209/3.3
• CPC Class Codes: B07C 5/3427 by changing or intensifying the optical properties prior to scanning, e.g. by inducing fluorescence under UV or x-radiation, subjecting the material to a chemical reaction
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/85 Investigating moving fluids or granular solids

Abstract

The invention generally relates to a novel method of sorting seed by providing a first seed population with a fluorescent indicator and a second seed population visually identical to the first seed population under standard operating conditions. The seed populations are combined to provide a combined seed population. A lamp having an output corresponding to the activation wavelength of the fluorescent indicator and a color sorting system are paired to count or separate the seed populations, as desired.

Claims

1. A method of sorting seeds comprising the steps of:

providing a first seed group having a preferred genetic characteristic;

providing a second seed group lacking the preferred genetic characteristic;

marking one of the first or second seed groups with an additive having an activatable color characteristic;

combining the first and second groups of seeds to create a combined seed group;

sampling the combined seed group;

activating the additive in the marked seed group within the combined seed group sample; and

segregating the seeds of the first group from the seeds of the second group in the sample by optical recognition of the activated color characteristic;

wherein the first and second seed groups are visually indistinct, wherein the additive comprises a fluorescent marker and the activatable color characteristic corresponds to the emission wavelength of the fluorescent marker, wherein the additive is activated by exposing the combined seed group sample to light corresponding to the activation wavelength of the fluorescent marker, and further comprising the step of placing the combined seed group sample on a background corresponding to the color of the non-fluorescent seeds when exposed to the light.

2. The method of claim 1 wherein the first and second seed groups are each treated with a seed coating and only one of the first or second seed groups is treated with the fluorescent dye.

3. The method of claim 2 wherein the seed coating comprises a fungicide.

4. The method of claim 2 wherein the seed coating comprises a pesticide.

5. The method of claim 1 wherein the fluorescent marker comprises a genetic marker.

6. The method of claim 5 wherein the first and second groups are substantially genetically identical except for the preferred genetic characteristic.

7. The method of claim 6 wherein the genetic marker is linked to the preferred genetic characteristic.

8. The method of claim 1 wherein the fluorescent marker comprises a chemical dye.

9. The method of claim 1 wherein the first and second groups are combined in a manner approximating a preferred ratio.

10. The method of claim 9 further comprising the step of determining the amount of seeds from the first and second groups of the sample.

11. The method of claim 10 further comprising the step of providing feedback to maintain the proper ratio of first and second groups in the combined group.

12. The method of claim 1 wherein the seed comprises maize.