Immobilized product tolerant microorganisms
First Claim
Patent Images
1. A system for making a solvent or organic acid, comprising a bioreactor that comprises:
- a) growth medium in contact with a solid or semi-solid support; and
b) product tolerant microorganisms of the Clostridium genus on the solid or semi-solid support, wherein said microorganisms produce the solvent or organic acid and have previously been mutagenized and selected for tolerance to the solvent or organic acid on a solid or semi-solid support in the presence of the solvent or organic acid, wherein the tolerance of the selected microorganisms to the solvent or organic acid is greater on a solid or semi-solid support than the tolerance to the solvent or organic acid exhibited when the selected microorganisms are cultured in a liquid media, and wherein the microorganisms have been selected to exhibit at least 125% tolerance to the solvent or organic acid in comparison to the tolerance of corresponding non-mutagenized microorganisms.
6 Assignments
0 Petitions
Accused Products
Abstract
Methods for mutagenizing and selecting microorganisms with increased product tolerance are provided. Additionally, methods and systems are disclosed for culturing the microorganisms to produce products.
107 Citations
38 Claims
-
1. A system for making a solvent or organic acid, comprising a bioreactor that comprises:
-
a) growth medium in contact with a solid or semi-solid support; and b) product tolerant microorganisms of the Clostridium genus on the solid or semi-solid support, wherein said microorganisms produce the solvent or organic acid and have previously been mutagenized and selected for tolerance to the solvent or organic acid on a solid or semi-solid support in the presence of the solvent or organic acid, wherein the tolerance of the selected microorganisms to the solvent or organic acid is greater on a solid or semi-solid support than the tolerance to the solvent or organic acid exhibited when the selected microorganisms are cultured in a liquid media, and wherein the microorganisms have been selected to exhibit at least 125% tolerance to the solvent or organic acid in comparison to the tolerance of corresponding non-mutagenized microorganisms. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
-
Specification