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Modified polymerases for improved incorporation of nucleotide analogues

  • US 8,460,910 B2
  • Filed: 09/10/2004
  • Issued: 06/11/2013
  • Est. Priority Date: 09/11/2003
  • Status: Active Grant
First Claim
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1. An altered 9°

  • N DNA polymerase, comprising the sequence of SEQ ID NO;

    14, whereinthe first amino acid of the motif A region is mutated to an amino acid selected from the group consisting of the aromatic amino acids, the amino acids with aliphatic side chains, glutamine (Q), cysteine (C), and serine (S);

    the second amino acid of the motif A region is mutated to an amino acid selected from the group consisting of alanine (A), serine (S), and glycine (G); and

    the third amino acid of the motif A region is mutated to an amino acid selected from the group consisting of serine (S), alanine (A), glycine (G), and the amino acids having beta-branched side chains,whereby the altered 9°

    N DNA polymerase is capable of incorporating a 3′

    O-azidomethyl-modified nucleotide.

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