Grass endophyte enhanced attributes

• US 8,465,963 B2
• Filed: 08/04/2006
• Issued: 06/18/2013
• Est. Priority Date: 08/16/2005
• Status: Active Grant

• Current Assignee: Grasslanz Technology Limited
• Original Assignee: Grasslanz Technology Limited
• Inventors: Rolston, Maurice Philip, Simpson, Wayne Roydon
• Primary Examiner(s): Afremova, Vera
• Application Number:
  US11/917,137
• Publication Number:
  US 20100278780A1
• Time in Patent Office: 2,510 Days
• Field of Search: None
• US Class Current: 435/254.1
• CPC Class Codes: A01H 15/00 Fungi; Lichens fungal microorganisms C12N1/14
• CPC Class Codes: A01H 17/00 Symbiotic or parasitic combinations including one or more new plants, e.g. mycorrhiza lichens A01H15/00
• CPC Class Codes: A01N 2300/00 Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
• CPC Class Codes: A01N 43/54 1,3-Diazines; Hydrogenated 1,3-diazines
• CPC Class Codes: A01N 43/653 1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
• CPC Class Codes: A01N 63/30 Microbial fungi; Substances produced thereby or obtained therefrom
• CPC Class Codes: C12N 1/145 Fungal isolates
• CPC Class Codes: C12R 2001/645 Fungi ; Processes using fungi

Abstract

Described is the method and use of AR584 endophyte (AGAL deposit no. NM98/04676 dated 12 May 1998) or variations in endophyte as exemplified by AR584, to produce a grass cultivar and AR584 combination wherein the plant or a part thereof produced by the combination retains viable AR584 endophyte after treatment with fungicide and/or after being subjected to elevated temperature or humidity.

Claims

1. A method of treating fungi infection in a grass plant whilst maintaining grass resistance to pests by the steps of:

(a) inoculating of grass seed with AR584 endophyte (AGAL deposit no. NM98/04676 dated 12 May

     1998);

(b) growing the grass and endophyte combination; and

(c) subjecting the grass to treatment with fungicide;

and wherein the grass retains resistance to pests after fungicide treatment.

2. The method of claim 1 wherein at least 50% endophyte viability is retained after fungicide treatment.

3. The method of claim 1 wherein the fungicide selected from:

the triazole group, the strobilurin group, and combinations of these two fungicide groups.

4. The method as claimed in claim 1 wherein the fungicide is applied at flowering of the grass plant.

5. The method as claimed in claim 1 wherein the grass is a tall fescue variety.

18. The method as claimed in claim 1 wherein at least 65% or greater endophyte viability is retained when the combination is sprayed with a 50:

50 wt/wt combination of triazole based fungicide and strobilurin based fungicide.

19. The method as claimed in claim 1 wherein the grass is a synthetic grass cultivar.

6. A method of maintaining or avoiding significant loss in endophyte viability in grass seed due to exposure of the seed to elevated temperature, elevated humidity or both, by the steps of:

(a) inoculating the grass with AR584 endophyte (AGAL deposit no. NM98/04676 dated 12 May

     1998);

(b) subjecting the grass to treatment with fungicide;

(c) collecting seeds from the inoculated grass; and

(d) subjecting the grass seed to elevated temperature, elevated humidity or both.

7. The method as claimed in claim 6 wherein greater than 50% endophyte viability is maintained in the grass seed.

8. The method as claimed in claim 6 wherein at least 50% viable endophyte is maintained after the seed is subjected to temperatures of between 15°

C. and 55°

C. for a time period of at least 3 days.

9. The method as claimed in claim 6 wherein the seed at least 50% viable endophyte is maintained after the seed is subjected to a relative humidity of 70% or greater for at least 3 days.

10. The method as claimed in claim 6 wherein the seed retains at least 60% endophyte viability after the seed is subjected to a temperature of up to 40°

C. and a relative humidity of up to 100% for at least a time period of 3 days.

11. The method as claimed in claim 6 wherein the grass is a tall fescue variety.

12. A method of maintaining or avoiding significant loss in endophyte viability during transportation of grass seed inoculated with endophyte by the steps of:

(a) inoculating the grass with AR584 endophyte (AGAL deposit no. NM98/04676 dated 12 May

     1998);

(b) subjecting the grass to treatment with fungicide;

(c) collecting seeds from the inoculated grass; and

(d) transporting the seeds, wherein the seeds are subjected to elevated temperatures, elevated humidity or both during transportation.

13. The method as claimed in claim 12 wherein greater than 50% endophyte viability is maintained in the grass seed after transportation.

14. The method as claimed in claim 12 wherein at least 50% viable endophyte is maintained after the seed is subjected to temperatures of between 15°

C. and 55°

C. for a time period of at least 3 days.

15. The method as claimed in claim 12 wherein at least 50% viable endophyte is maintained after the seed is subjected to a relative humidity of 70% or greater for at least 3 days.

16. The method as claimed in claim 12 wherein the seed retains at least 60% endophyte viability after the seed is subjected to a temperature of up to 40°

C. and a relative humidity of up to 100% for at least a time period of 3 days.

17. The method as claimed in claim 12 wherein the grass is a tall fescue variety.