Polypeptides, systems, and methods useful for detecting glucose
First Claim
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1. A biosensor for detecting a molecule of interest, comprising:
- an isolated polypeptide molecule capable of selectively-binding glucose, wherein the polypeptide molecule is selected from;
an unnatural analogue of the amino acid sequence of SEQ ID NO;
2; and
an unnatural analogue fragment of the amino acid sequence of SEQ ID NO;
2; and
a label associated with the polypeptide molecule;
wherein binding of glucose to the polypeptide molecule causes the label to generate a signal, such that the glucose can be detected.
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Abstract
The presently-disclosed subject matter provides biosensors for detecting molecules of interest. The biosensors include a polypeptide capable of selectively-binding glucose, wherein the polypeptide molecule is selected from: an unnatural analogue of wild type glucose binding protein; a fragment of wild type glucose binding protein; and an unnatural analogue fragment of wild type glucose binding protein.
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Citations
22 Claims
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1. A biosensor for detecting a molecule of interest, comprising:
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an isolated polypeptide molecule capable of selectively-binding glucose, wherein the polypeptide molecule is selected from;
an unnatural analogue of the amino acid sequence of SEQ ID NO;
2; and
an unnatural analogue fragment of the amino acid sequence of SEQ ID NO;
2; anda label associated with the polypeptide molecule; wherein binding of glucose to the polypeptide molecule causes the label to generate a signal, such that the glucose can be detected. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. An isolated polypeptide molecule capable of selectively-binding glucose, wherein the polypeptide molecule is selected from an unnatural analogue of the amino acid sequence of SEQ ID NO:
- 2; and
an unnatural analogue fragment of the amino acid sequence of SEQ ID NO;
2.
- 2; and
Specification