Detection and fluidic system of a flow cytometer
First Claim
1. A fluidic system for drawing a sample fluid, from a sample container, into an interrogation zone of a flow cytometer, comprising:
- a flow cell comprising a sheath fluid inlet and a sample fluid inlet coupled to the sample container, wherein the sheath fluid inlet and sample fluid inlet are coupled to a fluid path through the interrogation zone;
a sheath pump configured to pump a sheath fluid at a sheath flow rate through the sheath fluid inlet and into the interrogation zone by the fluid path;
a waste pump configured to pump a waste fluid at a waste flow rate from the interrogation zone, wherein a pressure differential resulting from the sheath flow rate and the waste flow rate draws the sample fluid, through the sample fluid inlet of the flow cell and into the interrogation zone, with the sheath fluid by the fluid path;
a detection system, coupled to the interrogation zone, configured to provide a data set of input signals from the sample fluid;
an analysis engine configured to recognize an aggregate particle event from the data set; and
a controller configured to automatically adjust a sample core stream diameter of the sample fluid in the interrogation zone, by adjusting the pressure differential, based on recognition of the aggregate particle event.
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Abstract
The fluidic system including a sheath pump that pumps sheath fluid from a sheath container into an interrogation zone, a waste pump that pumps waste fluid from the interrogation zone to a waste container, in which the flow rate of the sheath fluid is different from the flow rate of the waste fluid thereby drawing a sample fluid from a sample container into the interrogation zone, a detection system that provides a data set of input signals from the sample fluid, an analysis engine that recognizes aggregate particle events in the data set, and a controller that automatically adjusts the flow rate of the sample fluid into the interrogation zone based on the recognition of aggregate particle events, by controlling at least one of the flow rates of the sheath fluid and the waste fluid.
224 Citations
23 Claims
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1. A fluidic system for drawing a sample fluid, from a sample container, into an interrogation zone of a flow cytometer, comprising:
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a flow cell comprising a sheath fluid inlet and a sample fluid inlet coupled to the sample container, wherein the sheath fluid inlet and sample fluid inlet are coupled to a fluid path through the interrogation zone; a sheath pump configured to pump a sheath fluid at a sheath flow rate through the sheath fluid inlet and into the interrogation zone by the fluid path; a waste pump configured to pump a waste fluid at a waste flow rate from the interrogation zone, wherein a pressure differential resulting from the sheath flow rate and the waste flow rate draws the sample fluid, through the sample fluid inlet of the flow cell and into the interrogation zone, with the sheath fluid by the fluid path; a detection system, coupled to the interrogation zone, configured to provide a data set of input signals from the sample fluid; an analysis engine configured to recognize an aggregate particle event from the data set; and a controller configured to automatically adjust a sample core stream diameter of the sample fluid in the interrogation zone, by adjusting the pressure differential, based on recognition of the aggregate particle event. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method for drawing a sample fluid, from a sample container, into an interrogation zone of a flow cytometer, comprising:
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simultaneously pumping sheath fluid at a sheath flow rate, through a flow cell, into the interrogation zone and waste fluid at a waste flow rate from the interrogation zone, wherein the sample container is fluidically coupled to the flow cell, such that a pressure differential resulting from the sheath flow rate and the waste flow rate draws the sample fluid, from the sample container and into the interrogation zone; collecting a data set of input signals from the sample fluid; recognizing an aggregate particle event from the data set based on an event recognizing algorithm; and automatically adjusting a sample core stream diameter of the sample fluid in the interrogation zone, by adjusting the pressure differential, based on recognition of the aggregate particle event. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23)
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Specification