Method for removing antiplatelet agent and anticoagulant from a platelet composition by diafiltration

• US 8,470,521 B2
• Filed: 07/31/2012
• Issued: 06/25/2013
• Est. Priority Date: 06/15/2009
• Status: Expired due to Fees

• Current Assignee: Biovec Transfusion LLC
• Original Assignee: Biovec Transfusion LLC
• Inventors: Sehgal, Lakshman R.
• Primary Examiner(s): SAUCIER, SANDRA E
• Application Number:
  US13/563,360
• Publication Number:
  US 20120315619A1
• Time in Patent Office: 329 Days
• Field of Search: None
• US Class Current: 435/2
• CPC Class Codes: B01D 2313/66 Biodegradability of parts of the module
• CPC Class Codes: B01D 2315/16 Diafiltration
• CPC Class Codes: B01D 2323/39 Electrospinning
• CPC Class Codes: B01D 2325/04 Characteristic thickness
• CPC Class Codes: B01D 2325/20 Specific permeability or cut-off range
• CPC Class Codes: B01D 61/145 Ultrafiltration
• CPC Class Codes: B01D 61/243 Dialysis
• CPC Class Codes: B01D 61/246 Membrane extraction
• CPC Class Codes: B01D 63/02 Hollow fibre modules
• CPC Class Codes: B01D 69/02 characterised by their properties
• CPC Class Codes: B01D 69/08 Hollow fibre membranes manufacture of hollow fibres D01D5/24, D01F1/08

Abstract

The present invention relates to methods for removing antiplatelet agents and anticoagulants from a platelet preparation. In one embodiment, the method includes the step of flowing the platelet preparation through a filtering tube comprising a filtering membrane and separating the antiplatelet agents and anticoagulants from the platelet preparation by tangential flow filtration. In another embodiment, the method includes the step of passing the platelet preparation through porous material that specifically binds to the antiplatelet agents and anticoagulants.

Claims

1. A method for removing antiplatelet agents and anticoagulants from a platelet preparation using diafiltration, comprising:

circulating a platelet preparation comprising platelets, a short-to-ultra short acting antiplatelet agent comprising a short-to-ultrashort GPIIb/IIIA inhibitor, and a short-to-ultra short acting anticoagulant comprising a short-to-ultrashort acting factor Xa inhibitor through a hollow fiber membrane capable of separating the antiplatelet agent and the anticoagulant from the platelets,

wherein a diafiltration buffer is added to the platelet preparation during circulation to maintain a constant volume of the platelet preparation.

2. The method of claim 1, wherein the hollow fiber membrane comprises a material selected from the group consisting of regenerated cellulose, cellulose acetate, polyamide, polyurethane, polypropylene, polysulfone, polyethersulfone, polycarbonate, nylon, polyimide and combinations thereof.

3. The method of claim 1, wherein the hollow fiber membrane comprises polysulfone or polyethylsulfone.

4. The method of claim 1, wherein the hollow filter membrane has an inner diameter of at least 0.5 mm and a pore size ranging from molecular cut off of 3000 daltons to 0.5 micron.

5. The method of claim 1, wherein the platelet preparation flows through the hollow fiber membrane at a flow rate of 150 to 400 ml/min.

6. The method of claim 1, wherein the diafiltration buffer comprises 20% plasma.

7. The method of claim 1, wherein the platelet preparation is circulated through the hollow fiber membrane until a 10 to 15 volume exchange with the diafiltration buffer is achieved.