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Processing polynucleotide-containing samples

  • US 8,470,586 B2
  • Filed: 05/03/2005
  • Issued: 06/25/2013
  • Est. Priority Date: 05/03/2004
  • Status: Active Grant
First Claim
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1. A microfluidic device, comprising:

  • a processing region having a vertical central axis therethrough, the processing region comprised of an inlet, a retention member, a filter, and an outlet;

    said retention member being configured to preferentially retain one or more polynucleotides in a sample as compared to polymerase chain reaction inhibitors in the sample, wherein said retention member comprises a plurality of polynucleotide binding particles, and said plurality of binding particles having surfaces that comprise a poly-cationic polyamide bound thereto, further wherein the retention member is configured to retain polynucleotides from a sample preferentially to inhibitors in the sample as the sample passes through the retention member from the processing region inlet to the processing region outlet;

    wherein the vertical central axis of the processing region is perpendicular to a horizontal plane of the microfluidic device;

    wherein the processing region outlet is disposed along the vertical central axis above the processing region inlet;

    wherein the filter is configured to prevent the plurality of binding particles from passing downstream of the processing region;

    a device inlet in communication with, and upstream of, the processing region;

    a device outlet in communication with, and downstream of, the processing region; and

    a detection region in fluid communication with and downstream of the retention member.

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