Waveguide substrates and optical systems and methods of use thereof
First Claim
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1. An analytic device, comprising:
- a first substrate comprising a first surface;
two or more waveguides disposed upon or within the first substrate; and
,an analyte region disposed sufficiently proximal to a core of the at least one of the two or more waveguides to be illuminated by an evanescent field emanating from the core when optical energy is passed through the core, wherein the analyte region comprises a single optically resolvable immobilized complex.
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Abstract
This invention provides substrates for use in various applications, including single-molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided.
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Citations
29 Claims
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1. An analytic device, comprising:
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a first substrate comprising a first surface; two or more waveguides disposed upon or within the first substrate; and
,an analyte region disposed sufficiently proximal to a core of the at least one of the two or more waveguides to be illuminated by an evanescent field emanating from the core when optical energy is passed through the core, wherein the analyte region comprises a single optically resolvable immobilized complex. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 27, 28)
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11. An analytic device, comprising:
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a substrate comprising a first surface and at least a first optical waveguide disposed upon or proximal to the first surface of the substrate; a reflective or absorptive layer disposed over the first surface of the substrate; and an analyte region disposed within an aperture extending through the reflective or absorptive layer toward a core of the first optical waveguide, where the analyte region within the aperture is disposed sufficiently proximal to the core of the first optical waveguide to be illuminated by an evanescent field emanating from the core when optical energy is passed through the first optical waveguide, wherein passage of optical energy emanating from the evanescent field or the analyte region is mitigated by the reflective or absorptive layer. - View Dependent Claims (12, 13, 14, 15, 23, 24, 25, 26)
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16. An analytic device, comprising:
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a substrate comprising a detection region and at least one optical waveguide that traverses the detection region, wherein the at least one optical waveguide has a first end coupled to an optical energy source and a second end that is not coupled to the optical energy source, and further wherein the optical waveguide is configured to have a higher confinement of optical energy at the second end than at the first end; and a plurality of analyte regions disposed on a surface of the substrate in the detection region and sufficiently proximal to a core of the optical waveguide to be illuminated by an evanescent field emanating from the core when optical energy is passed through the optical waveguide. - View Dependent Claims (17, 18, 19, 20, 21, 29)
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22. A method for providing uniform illumination to a plurality of analyte regions on a substrate, the method comprising:
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a) disposing a waveguide core within a substrate, wherein the waveguide core is configured to gradually decrease a measure of optical confinement of the waveguide core; b) disposing the plurality of analyte regions along a portion of the substrate proximal to the waveguide core; and c) coupling excitation illumination into the waveguide core.
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Specification